- Clone
- RV3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD44 variant 9, CD44 variant isoform 9
- Isotype
- Rat IgG2a, κ
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Human prostatic carcinoma cell line, PC3 was stained with purified CD44 isoform 9 (clone RV3, filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE. -
Human paraffin-embedded colon cancer tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD44v9 (clone RV3) purified at 4°C overnight, followed by 2.5 µg/ml of anti-rat IgG (clone Poly4054) Alexa Fluor® 647 (red) for 2 hours at room temperature. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 394402 | 100 µg | $375.00 | |||
CD44 isoform 9, also known as CD44v9, is a CD44 isofom consisting of exons 1-5, 13 (v9) and 15 to 19, product of alternative splicing. CD44v9 is expressed by cancer stem cells, epithelial-type carcinomas, subsets of monocytes and subsets of lymphocytes; have a role in tumor initiation, maintenance and metastasis of cancer cells. CD44v9 interacts with the glutamate-cystine transporter xCT, increasing reduced glutathione and contributing to the ROS resistance of cancer cells, and is a potential predictive marker for recurrence of some cancers and a potential therapeutic target.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Human CD44v8-10 transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-P - Verified
IHC-F - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections3.
-
Application References
(PubMed link indicates BioLegend citation) -
- Kakehashi, A., et al. 2016. Cancer Sci. 107: 609. (IHC-P)
- Aso T, et al. 2015. PLoS One. 10: e0116596. (IHC-P)
- Yoshikawa M, et al. 2013. Cancer Res. 73: 1855. (IHC-F)
- Ishimoto T, et al. 2011. Cancer Cell. 387-400. (IHC-P)
- RRID
-
AB_2734487 (BioLegend Cat. No. 394402)
Antigen Details
- Distribution
-
Cancer stem cells, epithelial-type carcinomas, subset of monocytes, subset of lymphocytes.
- Function
- Role in tumor initiation and maintenance and metastasis of cancer cells, Interacts with the glutamate-cystine transporter xCT, increasing reduced glutathione and contributing to the ROS resistance of cancer cells.
- Cell Type
- Epithelial cells, Lymphocytes, Monocytes, Neutrophils
- Biology Area
- Cancer Biomarkers, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
- Hagiwara M, et al. 2018. BMC Cancer. 18:113
- Matsumoto T, et al. 2017. Oncogenesis. 6:397
- Bertaux-Skeirik N, et al. 2017. J. Pathol. 242:463
- Kodama H, et al. 2017. Br. J. Cancer. 116:186
- Gene ID
- 960 View all products for this Gene ID
- UniProt
- View information about CD44v9 on UniProt.org
Other Formats
View All CD44v9 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD44 variant 9 (CD44v9) | RV3 | FC,IHC-P,IHC-F |
| Alexa Fluor® 594 anti-human CD44 variant 9 (CD44v9) | RV3 | IHC-P |
| PE anti-human CD44 variant 9 (CD44v9) | RV3 | FC |
| Alexa Fluor® 647 anti-human CD44 variant 9 (CD44v9) | RV3 | FC,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD44 variant 9 (CD44v9)
Human prostatic carcinoma cell line, PC3 was stained with pu... 
Human paraffin-embedded colon cancer tissue slices were prep... -
Alexa Fluor® 594 anti-human CD44 variant 9 (CD44v9)
Human paraffin-embedded colon cancer tissue slices were prep... -
PE anti-human CD44 variant 9 (CD44v9)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
Alexa Fluor® 647 anti-human CD44 variant 9 (CD44v9)
PHA-stimulated (3 day) human peripheral blood lymphocytes we... 
Human paraffin-embedded colon cancer tissue slices were prep...