- Clone
- QA20A68 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Mouse IgG1, κ
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Human peripheral blood lymphocytes stained with purified anti-human CD45RB recombinant (clone QA20A68) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 377702 | 100 µg | $112.00 | |||
CD45RB is a 180-240 kD single chain type I membrane glycoprotein. It is an exon 5 splice variant of the tyrosine phosphatase CD45. The CD45RB isoform is expressed on thymocytes, T cell subsets, B cells, monocytes/macrophages, granulocytes, and dendritic cells. CD45RB enhances both T cell receptor and B cell receptor signaling mediated activation. CD45RB negatively regulates protein kinase activity, and negatively regulates cytokine-mediated signaling pathway.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Clone QA20A68 can completely block the binding of MEM-55 to target cells.
- RRID
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AB_2904411 (BioLegend Cat. No. 377702)
Antigen Details
- Structure
- Tyrosine phosphatases, type I transmembrane, 180-240 kD (exon 5 splicing of CD45 gene)
- Distribution
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Highly expressed in bone marrow, colon, lung, and most normal epithelial cells. Also highly expressed on carcinomas of gastrointestinal origin. Expressed during early erythrogenesis.
- Function
- Enhances TCR and BCR signaling. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
- Interaction
- Human
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Cell Type
- B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, T cells, Thymocytes, Tregs
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
- Thomas ML. 1989. Annu Rev Immunol. 7:339-69.
- Trowbridge IS, et al. 1994. Annu Rev Immunol.12:85-116.
- Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45RB on UniProt.org
Other Formats
View All CD45RB Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD45RB Recombinant Antibody | QA20A68 | FC |
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Purified anti-human CD45RB Recombinant Antibody
Human peripheral blood lymphocytes stained with purified ant...