Purified anti-human CD46 Antibody

Pricing & Availability
Clone
TRA-2-10 (See other available formats)
Regulatory Status
RUO
Other Names
Complement membrane cofactor protein, measles virus receptor, MCP
Isotype
Mouse IgG1, κ
TRA-2-10_Pure_071211
Human peripheral blood lymphocytes were stained with purified CD46 (clone TRA-2-10) (filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
  • TRA-2-10_Pure_071211
    Human peripheral blood lymphocytes were stained with purified CD46 (clone TRA-2-10) (filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
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352403 25 µg $89.00
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352404 100 µg $206.00
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Description

CD46 is a transmembrane protein that is known as a complement membrane cofactor protein, MCP, and measles virus receptor. It is widely expressed on leukocytes, platelets, epithelial cells, and fibroblasts. Multiple isoforms of CD46 have been reported with molecular weights ranging from 45-75 kD. CD46 binds complement components C3b and C4b and has been reported to play a role in T cell regulation. In addition to the complement components, CD46 has been shown to interact with moesin, c-Src, tetraspanin 4, c-Yes, and integrin β1. CD46 is also known to be a cellular receptor for human measles virus and human herpes virus-6 as well as other human pathogens, such as Streptococcus pyogenes. Defects in CD46 have been associated with hemolytic-uremic syndrome.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
2102Ep human embryonal carcinoma cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence staining1, immunoprecipitation1, and blocking of measles virus infection2. The epitope of TRA-2-10 is in the SCR-1 domain of CD46.

Application References

(PubMed link indicates BioLegend citation)
  1. Cardone J, et al. 2010. Nat. Immunol. 11:862. (IF, IP)
  2. Blau DM, Compans RW. 1995. Virology. 210:91. (Block)
Product Citations
  1. Kuppan JP, et al. 2021. Elife. 10:. PubMed
RRID
AB_10900243 (BioLegend Cat. No. 352403)
AB_10900243 (BioLegend Cat. No. 352404)

Antigen Details

Structure
Transmembrane receptor, contains 4 SUSHI domains, multiple isoforms exist ranging in molecular weight from 45-75 kD. Larger isoforms from 65-75 kD are not expressed on peripheral blood cells.
Distribution

Widely expressed on leukocytes, platelets, epithelial cells, and fibroblasts.

Function
Binds complement components C3B and C4B with cofactor activity for the I-dependent cleavage of these proteins. May also play a role in T cell regulation.
Interaction
Human 55-73 kD polypeptide encoded by human chromosome 1.
Cell Targets
Moesin, c-Src, complement component 3, complement component 4B, tetraspanin 4, c-Yes, Integrin β1
Receptors
C3B, C4B, Measles virus, Human herpes virus-6
Biology Area
Cell Biology, Complement, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Cardone J, et al. 2010. Nat. Immunol. 11:862.
2. Dorig RE, et al. 1993. Cell 75:295.
3. Kemper C, et al. 2003. Nature 421:388.
4. Frecha C, et al. 2011. J. Virol. 85:5975.
5. Adams EM, et al. 1991. J. Immunol. 147:3005.

Gene ID
4179 View all products for this Gene ID
UniProt
View information about CD46 on UniProt.org
Go To Top Version: 4    Revision Date: 06/09/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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