Purified anti-human CD54 Antibody

Pricing & Availability
Clone
HA58 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
ICAM-1, Ly-47
Isotype
Mouse IgG1, κ
HA58_PE_090711
Human peripheral blood lymphocytes were stained with CD54 (clone HA58) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • HA58_PE_090711
    Human peripheral blood lymphocytes were stained with CD54 (clone HA58) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • HA58_Purified_CD54_Antibody_ICC_011821
    MDA-MB435 breast cancer cell line was stained with anti-human CD54, detected with anti-mouse DyLight™ 649, and nuclear counterstained with DAPI. Images were acquired with a TE300 fluorescence microscope with a 20x objective. Data provided by: Er Liu and John Nolan, La Jolla Bioengineering Institute
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353101 25 µg $54.00
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353102 100 µg $114.00
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Description

CD54 is a 85-110 kD type I transmembrane protein also known as ICAM-1. It is expressed on activated endothelial cells, high endothelial venules, T and B cells, monocytes/macrophages, granulocytes, and dendritic cells. The expression of ICAM-1 can be released from the cell surface. CD54 plays a role in cellular adhesion and is involved in inflammation and leukocyte extravasation. CD54 has also been shown to be the major cellular receptor for rhinovirus. ICAM-1 binds to CD11a/CD18 (LFA-1), CD11b/CD18 (Mac-1), CD11c/CD18 (p150, 95) as well as hyaluronan and fibrinogen.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Colonic cancer BM314 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC - Verified
IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone HA58 recognizes an epitope located in the extracellular D1 domain of CD543. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Tsujisaki M, et al. 1991. Clin. Exp. Immunol. 85:3.
  2. Kanwar JR, et al. 2003. Cancer Gene Ther. 10:468.
  3. Kohka H, et al. 1998. J. Leukoc. Biol. 64:519.
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Zhang W, et al. 2022. Dev Cell. 57:329. PubMed
  2. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  3. Behrens NE, et al. 2019. MBio. 10:2. PubMed
  4. Sawada J, et al. 2021. Am J Pathol. 191:396. PubMed
  5. Poventud-Fuentes I, et al. 2021. Small. 17:e2004889. PubMed
  6. Hunter S, et al. 2018. J Hepatol. 69:654. PubMed
  7. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  8. Vazquez Rodriguez G, et al. 2017. Cancer Immunol Res. 10.1158/2326-6066.CIR-16-0150. PubMed
  9. Kim N, et al. 2020. Cancers (Basel). 12:. PubMed
  10. Eriksson E, et al. 2017. Clin Cancer Res. 5.018055556. PubMed
  11. Gunasekaran M, et al. 2018. J Immunol. 200:2535. PubMed
  12. Nishihara H, et al. 2021. STAR Protocols. 2(2):100563. PubMed
RRID
AB_11204422 (BioLegend Cat. No. 353101)
AB_11204422 (BioLegend Cat. No. 353102)

Antigen Details

Structure
Type I membrane protein, Ig superfamily, 85-110 kD
Distribution

Endothelial cells, T cells and B cells, monocytes/macrophages, granulocytes, and dendritic cells

Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Voraberger G, et al. 1991. J. Immunol. 147:2777.
2. Staunton DE, et al. 1988. Cell 52:925.
3. Greve JM, et al. 1989. Cell 56:839.

Gene ID
3383 View all products for this Gene ID
UniProt
View information about CD54 on UniProt.org
Go To Top Version: 4    Revision Date: 01/18/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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