- Clone
- W22098E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Leu-19, NKH1, NCAM-1
- Isotype
- Mouse IgG2b, κ
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Human peripheral blood lymphocytes were stained with anti-human CD16 (clone 3G8) APC and purified anti-human CD56 (NCAM) (clone W22098E) (left) or purified mouse IgG2b, κ isotype control (right), followed by anti-mouse IgG PE.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 390352 | 100 µg | $115.00 | |||
CD56, also known as NCAM (neural cell adhesion molecule), Leu-19, or NKH1, is a single transmembrane glycoprotein. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK and NKT cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparan sulfate.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Mammalian-derived recombinant protein aa 20-718
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Flow cytometry testing showed that clone W22098E can block the binding of clones HCD56 and W22097A on target cells.
Flow cytometry testing showed that clone W22098E does not affect the binding of clones MEM-188, 5.1H11 and W22098A on target cells. - RRID
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AB_3662359 (BioLegend Cat. No. 390352)
Antigen Details
- Structure
- Ig superfamily, single transmembrane or GPI-anchored glycoprotein
- Distribution
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NK cells, T subset, neural tissue, some LGL and myeloid leukemias
- Function
- Adhesion
- Ligand/Receptor
- Heparan sulfate
- Cell Type
- B cells, Leukemia, Mesenchymal Stem Cells, Neurons, T cells
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
- Lanier L, et al. 1991. J Immunol. 146:4421-6.
- Hemperly J, et al. 1990. J Mol Neurosci. 2:71-8.
- Cremer H, et al. 1994. Nature. 367:455-9.
- Gene ID
- 4684 View all products for this Gene ID
- UniProt
- View information about CD56 on UniProt.org
Other Formats
View All CD56 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD56 (NCAM) | W22098E | FC |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-human CD56 (NCAM)
Human peripheral blood lymphocytes were stained with anti-hu...