- Clone
- QA18A42 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Mouse IgG1, κ
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Human peripheral blood lymphocytes were stained with purified anti-human CD62L recombinant (clone QA18A42) (left) or purified mouse IgG1, κ isotype control (right) followed by goat anti-mouse IgG PE. Cells were co-stained with CD3 APC.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 306902 | 100 µg | $101.00 | |||
CD62L is a 74-95 kD single chain type I glycoprotein referred to as L-selectin or LECAM-1. It is expressed on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. CD62L is important for the homing of naïve lymphocytes to high endothelial venules in peripheral lymph nodes and Peyer's patches. It also plays a role in leukocyte rolling on activated endothelial cells.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Immunogen
- Concentrated supernatant from PMA-activated human peripheral blood leukocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
QA18A42 is able to completely block the binding of clone DREG-56 on target cells.
- RRID
-
AB_2888837 (BioLegend Cat. No. 306902)
Antigen Details
- Structure
- Selectin, single chain glycoprotein, 74-95 kD
- Distribution
-
Majority of B cells, naïve T cells, subset of memory T and NK cells, monocytes, granulocytes, thymocytes
- Function
- Leukocyte homing, leukocyte tethering, rolling
- Ligand/Receptor
- CD34, GlyCAM, MAdCAM-1
- Cell Type
- B cells, Granulocytes, Leukocytes, Lymphocytes, Monocytes, Neutrophils, NK cells, NKT cells, T cells
- Biology Area
- Adaptive Immunity, Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Kishimoto T, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
2. Kishimoto T, et al. 1991. Blood 78:805 - Gene ID
- 6202 View all products for this Gene ID
- UniProt
- View information about CD62L on UniProt.org
Other Formats
View All CD62L Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD62L Recombinant Antibody | QA18A42 | FC |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-human CD62L Recombinant Antibody
Human peripheral blood lymphocytes were stained with purifie...