Purified anti-human CD66b Recombinant Antibody

Pricing & Availability
Clone
QA17A51 (See other available formats)
Regulatory Status
RUO
Other Names
CD67, CGM6, NCA-95, CEACAM8
Isotype
Mouse IgG1, κ
QA17A51_PURE_CD66b_Antibody_070219
Human peripheral blood granulocytes were stained with CD66b (clone QA17A51) Purified (filled histogram) or Mouse IgG1, κ Purified isotype control (open histogram) followed by anti-mouse IgG1 PE
  • QA17A51_PURE_CD66b_Antibody_070219
    Human peripheral blood granulocytes were stained with CD66b (clone QA17A51) Purified (filled histogram) or Mouse IgG1, κ Purified isotype control (open histogram) followed by anti-mouse IgG1 PE
  • QA17A51_PURE_CD66b_Antibody_2_022520
    Human paraffin-embedded spleen tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with 1X Citric buffer pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD66b (clone QA17A51) antibody overnight at 4°C. On the next day, tissue was incubated with Alexa Fluor® 594 Goat anti-mouse IgG (clone poly4053) antibody (red). Nuclei were counter-stained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
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Cat # Size Price Quantity Check Availability
396902 100 µg $141.00
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Description

CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814367 (BioLegend Cat. No. 396902)

Antigen Details

Distribution

Granulocytes/neutrophils

Function
Cell adhesion, neutrophil activation
Ligand/Receptor
CD66b
Cell Type
Granulocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Kuijpers T, et al. 1993. J. Immunol. 151:4934
  2. Kuroki M, et al. 1992. J. Leuk. Biol. 52:551
Gene ID
1088 View all products for this Gene ID
UniProt
View information about CD66b on UniProt.org
Go To Top Version: 2    Revision Date: 02/25/2020

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