- Clone
- 1D4/CD66c (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CEACAM 6
- Isotype
- Mouse IgG3, κ
-
Human lysed washed granulocytes stained with purified anti-human CD66c (clone 1D4/CD66c) (filled histogram) or mouse IgG1, κ isotype control purified (open histogram) followed by anti-mouse IgG PE. -
Human colon cancer cell line HT-29 stained with purified anti-human CD66c (clone 1D4/CD66c) (filled histogram) or mouse IgG1, κ isotype control purified (open histogram) followed by anti-mouse IgG PE. -
Human paraffin-embedded spleen tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD66c (clone 1D4/CD66c) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with goat anti-mouse secondary antibody (clone Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was captured with a 10X objective. -
Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD66c (clone 1D4/CD66c) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with goat anti-mouse secondary antibody (clone Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was captured with a 10X objective.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 365002 | 100 µg | $147.00 | |||
CD66c, also known as CEACAM6, is one of the splice variants of CD66. It is expressed on endothelial, epithelial cells, neutrophils, monocytes, and myeloma and cancer cells, such as B-cell acute lymphoblastic lymphomas. CD66 expression level can be induced on T cells, B cells, and CD16-negative NK cells. Like the other CD66 splice variants, CD66c is involved in cell adhesion and growth, neutrophil activation, and signaling. It plays a role in hemophilic and heterophilic adhesion.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2904392 (BioLegend Cat. No. 365002)
Antigen Details
- Structure
- Member of the CEA (carcinoembryonic antigen) family of the Ig superfamily
- Distribution
-
Granulocytes, epithelial cells
- Function
- Homophilic and heterophilic adhesion
- Ligand/Receptor
- CD66a-e, CD62E and Galectins
- Cell Type
- Epithelial cells, Granulocytes
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
- Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiley-Liss. A John Wiley & Sons Inc. Publication
- Gene ID
- 4680 View all products for this Gene ID
- UniProt
- View information about CD66c on UniProt.org
Other Formats
View All CD66c Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD66c | 1D4/CD66c | FC,IHC-P |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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