Purified anti-human CD70 Antibody

Pricing & Availability
Clone
113-16 (See other available formats)
Regulatory Status
RUO
Other Names
CD27 ligand, CD27L, Ki-24 antigen, Tumor necrosis factor ligand superfamily, member 7 (TNFSF7)
Isotype
Mouse IgG1, κ
113-16_Pure_070212
Human myeloma cell line, U266, was stained with purified CD70 (clone 113-16, filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • 113-16_Pure_070212
    Human myeloma cell line, U266, was stained with purified CD70 (clone 113-16, filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
Compare all formats
Cat # Size Price Quantity Check Availability
355101 25 µg $65.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
355102 100 µg $136.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD70, also known as CD27L, is a 50 kD type II transmembrane glycoprotein and member of the tumor necrosis factor superfamily. CD70 is expressed on activated T, B and NK cells, activated plasmacytoid dendritic cells (pDCs), and chronic B cell lymphocytic leukemia and large B cell lymphomas. CD70 costimulates T cell proliferation and differentiation. It plays a role in the pDC-induced B cell differentiation. The ligand of CD70 is CD27.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CD70-transfected L cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications for the relevant formats include: blocking of plasmacytoid dendritic cell induced B cell proliferation and Ig secretion1.

Application References

(PubMed link indicates BioLegend citation)
  1. Shaw J, et al. 2010. Blood 115:3051. (Block)
Product Citations
  1. Gabitova-Cornell L, et al. 2020. Cancer Cell. 38(4):567-583.e11. PubMed
  2. Zhang X, et al. 2022. Acta Biochim Biophys Sin (Shanghai). 54:1518. PubMed
  3. Tian Y, et al. 2019. Cell Rep. 29:4482. PubMed
  4. Francescone R, et al. 2020. Cancer Discovery. 11(2):446-479. PubMed
  5. Abolhassani H, et al. 2017. J Exp Med . 214(1):91-106. PubMed
  6. Ravell JC, et al. 2020. J Clin Invest. 130:507. PubMed
  7. Martin E, et al. 2020. JCI Insight. :5. PubMed
RRID
AB_2561429 (BioLegend Cat. No. 355101)
AB_2561429 (BioLegend Cat. No. 355102)

Antigen Details

Structure
Type II transmembrane glycoprotein, member of the tumor necrosis factor superfamily, 50 kD
Distribution

Activated T cells and B cells, activated NK cells, activated plasmacytoid dendritic cells, some B cell lymphomas

Function
Role in T cell proliferation, generation of cytolytic and memory T cells, B cell proliferation and differentiation
Ligand/Receptor
CD27
Cell Type
B cells, Dendritic cells, NK cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Bowman MR, et al. 1994. J. Immunol. 152:1756.
2. Shaw J, et al. 2010. Blood 115:3051.
3. Keller AM, et al. 2009. Blood 113:5167.

Gene ID
970 View all products for this Gene ID
UniProt
View information about CD70 on UniProt.org
Go To Top Version: 2    Revision Date: 06/15/2022

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account