Purified anti-human CD72 Antibody

Pricing & Availability
Clone
3F3 (See other available formats)
Regulatory Status
RUO
Workshop
VI CD72.1
Other Names
Lyb-2, Ly-19.2, Ly-32.2
Isotype
Mouse IgG2b, κ
3F3_Purified_070507
Human peripheral blood lymphocytes stained with purified 3F3,followed by anti-mouse IgGs FITC
  • 3F3_Purified_070507
    Human peripheral blood lymphocytes stained with purified 3F3,followed by anti-mouse IgGs FITC
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Cat # Size Price Quantity Check Availability
316202 100 µg $112.00
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Description

CD72 is a 39-43 kD type II membrane glycoprotein. It is a disulfide-linked homodimer belonging to C-type lectin family. CD72 is a pan-B cell marker expressed on pre-pre-B cells throughout B cell differentiation with the exception of plasma cells. It is also expressed on follicular dendritic cells, splenic red pulp macrophages (but not on peripheral blood monocytes), and liver Kupffer cells. CD72, a negative coreceptor of B cells, contains immunoreceptor tyrosine-based inhibitory motifs in the cytoplasmic domain which has been shown to recruit the tyrosine phosphatase SHP-1. Ligation of CD72 with its ligand regulates CD72 tyrosine dephosphorylation and SHP-1 dissociation to promote B cell activation and proliferation. CD100 and CD5 have been shown to be CD72 ligands. The CD100-CD72 interaction plays a role in maintenance of B cell homeostasis.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
Product Citations
  1. Glass MC, et al. 2022. Cell Rep. 39:110728. PubMed
RRID
AB_2260104 (BioLegend Cat. No. 316202)

Antigen Details

Structure
Type II membrane glycoprotein, C-type lectin family, 39-43 kD, homodimer
Distribution

Pre-pre-B cells throughout B cells differentiation (but not on plasma cells), follicular dendritic cells, splenic red pulp macrophages, liver Kupffer cells

Function
negative regulation of B cell receptor signaling
Ligand/Receptor
CD5, CD100/Sema4D
Cell Type
B cells, Dendritic cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Schwarting T, et al. 1992. Am. J. Hematol. 41:151.
  3. Wu HJ and S. Bondata. 2002. Immunol. Res. 25:155.
  4. Kumanogoh A, et al. 2000. Immunity 13:621.
  5. Parnes JR and C. Pan. 2000. Immunol. Rev. 176:75.
  6. Kumanogoh A, et al. 2005. Intnl. Immunol. 17:1277.
Gene ID
971 View all products for this Gene ID
UniProt
View information about CD72 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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