Purified anti-human CD86 Antibody

Pricing & Availability
Clone
BU63 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
B7-2, B70, Ly-58
Isotype
Mouse IgG1, κ
BU63_PURE_CD86_Antibody_1_102424
Human peripheral blood monocytes stained with CD14 APC/Cyanine7 and purified human CD86 (clone BU63, top) or purified Mouse IgG1, κ isotype control (bottom), followed by anti-mouse IgG1 PE secondary antibody.
  • BU63_PURE_CD86_Antibody_1_102424
    Human peripheral blood monocytes stained with CD14 APC/Cyanine7 and purified human CD86 (clone BU63, top) or purified Mouse IgG1, κ isotype control (bottom), followed by anti-mouse IgG1 PE secondary antibody.
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Cat # Size Price Quantity Check Availability
374202 100 µg $176.00
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Description

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is the ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce costimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can bind to CD152 as well, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
ARH 77 (B lymphoblastoid cell line).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Blocking, IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Ji T, et al. 2023. Oncogenesis. 12:31. PubMed
  2. Ceprián N, et al. 2021. Front Med (Lausanne). 8:705159. PubMed
  3. Song S, et al. 2021. Commun Biol. 4:1338. PubMed
  4. Hu G, et al. 2021. Nat Commun. 12:773. PubMed
RRID
AB_2687347 (BioLegend Cat. No. 374202)

Antigen Details

Structure
Ig superfamily, single-chain transmembrane glycoprotein, 80 kD
Distribution

Monocytes/macrophages, activated B cells and T cells, dendritic cells

Function
T Cell activation
Interaction
C-Jun, NFκB
Ligand/Receptor
CD28, CD152
Cell Type
B cells, Dendritic cells, Macrophages, Monocytes, T cells
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Hathcock K, et al. 1996. Adv. Immunol. 62:131. 
2. June C, et al. 1994. Immunol. Today 15:321.

Gene ID
942 View all products for this Gene ID
UniProt
View information about CD86 on UniProt.org
Go To Top Version: 1    Revision Date: 07/03/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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