- Clone
- S22015F (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Thy-1, Thy1
- Isotype
- Mouse IgG1, κ
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HEL cells (Human erythroleukemia cell line) were stained with purified anti-human CD90 (Thy1) (clone S22015F) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by goat anti-mouse IgG PE. -
IHC staining of purified anti-human CD90 (Thy1) (clone S22015F) on formalin-fixed paraffin-embedded human kidney (A) or tonsil (B, negative control) tissue. After antigen retrieval using 1X Citrate Buffer (Cat. No. 420902), the tissues were incubated with 10 μg/mL of purified anti-human CD90 (Thy1) (clone S22015F) at 4°C overnight, followed by incubation with 2.5 μg/mL of Alexa Fluor® 594 Goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 10X objective.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 389902 | 100 µg | $170.00 | |||
CD90, also known as Thy-1, is a 25-35 kD GPI-anchored protein. It belongs to the Ig superfamily. Human CD90 is expressed on neuronal cells, a subset of CD34+ cells, a subset of fetal liver cells and fetal thymocytes, fibroblasts, activated endothelial cells, and some leukemia cell lines. CD34+CD90+ cells are primitive hematopoietic stem cells. It has been reported that Thy-1 binds with β2 and β3 integrins and plays bimodal roles in the regulation of cell adhesion and neurite outgrowth, and inhibits hematopoietic stem cells proliferation and differentiation.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human CD90-transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration of 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone S22015F does not block the binding of clones S22015B nor 5E10 on target cells.
- RRID
-
AB_3106022 (BioLegend Cat. No. 389902)
Antigen Details
- Structure
- 25-35 kD glycoprotein, Ig superfamily
- Distribution
-
Subset of CD34+ hematopoietic stem cells, subset of fetal thymocytes, subset of fetal liver cells, fibroblast, activated endothelial cells, neurons, and some leukemia cell lines
- Function
- Cell adhesion
- Cell Type
- Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Leukemia, Neurons, Thymocytes
- Biology Area
- Cell Adhesion, Neurodegeneration, Neuroscience, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
- McKenzie JL, et al. 1981. J Immunol. 126:843-50.
- Avalos AM, et al. 2002. Biol Res. 35:231-8.
- Wetzel A, et al. 2004. J Immunol. 172:3850-9.
- Gene ID
- 7070 View all products for this Gene ID
- UniProt
- View information about CD90 on UniProt.org
Other Formats
View All CD90 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD90 (Thy1) | S22015F | FC,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-human CD90 (Thy1)
HEL cells (Human erythroleukemia cell line) were stained wit... 
IHC staining of purified anti-human CD90 (Thy1) (clone S2201...