Purified anti-human CD90 (Thy1) Antibody

Pricing & Availability
Clone
5E10 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
Thy-1, Thy1
Isotype
Mouse IgG1, κ
5E10_Pure_021508
Human erythroleukemic cell line HEL stained with purified 5E10, followed by anti-mouse IgG FITC
  • 5E10_Pure_021508
    Human erythroleukemic cell line HEL stained with purified 5E10, followed by anti-mouse IgG FITC
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Cat # Size Price Quantity Check Availability
328101 25 µg $83.00
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328102 100 µg $176.00
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Description

CD90 is a 25-35 kD GPI-anchored protein, also known as Thy-1. It belongs to the Ig superfamily. Human CD90 is expressed on neuronal cells, a subset of CD34+ cells, a subset of fetal liver cells and fetal thymocytes, fibroblasts, activated endothelial cells, and some leukemia cell lines. CD34+CD90+ cells are primitive hematopoietic stem cells. It has been reported that Thy-1 binds with β2 and β3 integrins and plays bimodal roles in the regulation of cell adhesion and neurite outgrowth, and inhibits hematopoietic stem cells proliferation and differentiation.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Pigtailed Macaque, Rhesus, Pig
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
HEL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
IHC-F, ICC, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 5E10 recognizes an epitope on Thy-1 independent of its glycosylation, but is abolished under reducing conditions.4 Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation1, and immunofluorescence3.

Application References

(PubMed link indicates BioLegend citation)
  1. Craig W, et al. 1993. J. Exp. Med. 177:1331. (IP)
  2. Gundlach CW 4th, et al. 2011. Bioconjug. Chem. 22:1706. (Pig Reactivity)
  3. Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF)
  4. Bradley JE, et al. 2013. Lab Invest. 93:365. (Epitope)
  5. Donnenberg VS, et al. 2010. Cytometry B. Clin. Cytom. 5:287. (IHC)
Product Citations
  1. Alpert A, et al. 2022. Cell Syst. 13:71. PubMed
  2. Chandler A, et al. 2022. J Orthop Res. 40:1918. PubMed
  3. Mendt M, et al. 2018. JCI Insight. 9:1523. PubMed
  4. Tornack J, et al. 2017. PLoS One. 12:e0169119. PubMed
  5. Stice SL, et al. 2022. Environ Health Perspect. 130:117002. PubMed
  6. Slamecka J, et al. 2017. Cell Cycle. 17:330. PubMed
  7. Floy ME, et al. 2022. STAR Protoc. 3:101275. PubMed
  8. Fitzgerald W, et al. 2018. Am J Reprod Immunol. 80:e12860. PubMed
  9. Azari S, et al. 2021. MBio. 12:e0184921. PubMed
  10. Gañán-Gómez I, et al. 2022. Nat Med. . PubMed
  11. Lin J, et al. 2019. J Immunol Res. 2019:6929286. PubMed
  12. Han L, et al. 2019. Haematologica. 10.3324/haematol.2018.205534. PubMed
  13. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
  14. Ran LJ, et al. 2018. Mol Med Rep. 18:723. PubMed
  15. Crawford LB, et al. 2021. J Virol. 95:. PubMed
  16. Li Z, et al. 2020. J Clin Lab Anal. 34:e23155. PubMed
RRID
AB_940390 (BioLegend Cat. No. 328101)
AB_940390 (BioLegend Cat. No. 328102)

Antigen Details

Structure
25-35 kD glycoprotein, Ig superfamily
Distribution

Subset of CD34+ hematopoietic stem cells, subset of fetal thymocytes, subset of fetal liver cells, fibroblast, activated endothelial cells, neurons and some leukemia cell lines

Function
Regulate hematopoiesis and neural cell growth, cell adhesion
Ligand/Receptor
β3 integrin, β2 integrin
Cell Type
Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Leukemia, Mesenchymal Stem Cells, Neurons, Thymocytes
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. McKenzie JL, et al. 1981. J. Immunol. 126:843.
2. Avalos AM, et al. 2002. Biol. Res. 35:231.
3. Wetzel A, et al. 2004. J. Immunol. 172:3850.

Gene ID
7070 View all products for this Gene ID
UniProt
View information about CD90 on UniProt.org
Go To Top Version: 4    Revision Date: 08/30/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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