Purified anti-human CD94 Antibody

Pricing & Availability
Clone
DX22 (See other available formats)
Regulatory Status
RUO
Other Names
KP43
Isotype
Mouse IgG1, κ
DX22_Purified_070507
Human peripheral blood lymphocytes stained with purified DX22, followed by anti-mouse IgGs FITC
  • DX22_Purified_070507
    Human peripheral blood lymphocytes stained with purified DX22, followed by anti-mouse IgGs FITC
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305502 100 µg $101.00
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Description

CD94 is a 43 kD type II transmembrane glycoprotein also known as KP43. CD94 belongs to the C-type lectin superfamily and is present as a covalently linked heterodimer with NKG2 on the cell surface. CD94 is expressed by NK cells, a subset of γδ T cells, and NKT cells. The CD94/NKG2A complex serves as an inhibitory receptor specific for HLA-class I molecules.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation4, inhibition of NK cell-mediated lysis5, immunohistochemical staining of acetone-fixed frozen tissue sections, and spatial biology (IBEX)6,7.

Application References

(PubMed link indicates BioLegend citation)
  1. Mizuki M, et al. 2000. Sarcoidosis Vasc. Diffuse Lung Dis. 17:54.
  2. Phillip J, et al. 1996. Immunity 5:163.
  3. Lazetic S, et al. 1996. J. Immunol. 157:4741.
  4. Lanier LL, et al. 1998. Immunity 8:693.
  5. Wooden SL, et al. 2005. J. Immunol. 175:1383.
  6. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  7. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Shao D, et al. 2008. J Leukoc Biol. 83:1323. PubMed
  2. Kato M, et al. 2021. PLoS One. 16:e0252116. PubMed
  3. Abd Hamid M et al. 2019. Cancer Immunol Res. 7(8):1293-1306 . PubMed
RRID
AB_314532 (BioLegend Cat. No. 305502)

Antigen Details

Structure
C-type lectin, type II transmembrane glycoprotein, covalently associates with NKG2, 43 kD
Distribution

NK cells , T subset

Function
Inhibits NK function
Cell Type
NK cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Lopez-Botet M, et al. 1997. Immunol. Rev. 155:165.
2. Moretta A, et al. 1997. Immunol. Rev. 155:105.

Gene ID
3824 View all products for this Gene ID
UniProt
View information about CD94 on UniProt.org
Go To Top Version: 2    Revision Date: 08/25/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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