Purified anti-human Cytokeratin 7 Antibody

Pricing & Availability
Clone
W16155A (See other available formats)
Regulatory Status
RUO
Other Names
Keratin type II cytoskeletal 7, Cytokeratin-7, CK-7, Keratin-7, K7, Type-II keratin Kb7, Sarcolectin, SCL, Cytokeratin 7, Keratin 7
Isotype
Rat IgG2a, κ
W16155A_Pure_Cytokeratin7_1_WB_Antibody_081717
Total cell lysate (15ug protein) from HaCaT, A549, MCF-7 (negative control) and NIH3T3 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:250 (2 µg/mL) diluted purified anti-KRT7 antibody (clone W16155A) or competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP rat-IgG secondary antibody conjugated to HRP (clone Poly4054) for clone W16155A or 1:3000 diluted donkey anti-rabbit IgG Antibody conjugated to HRP (clone Poly4064) for competitor’s antibody (upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower). Lane M: MW ladder, M* indicates longer exposure.
  • W16155A_Pure_Cytokeratin7_1_WB_Antibody_081717
    Total cell lysate (15ug protein) from HaCaT, A549, MCF-7 (negative control) and NIH3T3 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:250 (2 µg/mL) diluted purified anti-KRT7 antibody (clone W16155A) or competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP rat-IgG secondary antibody conjugated to HRP (clone Poly4054) for clone W16155A or 1:3000 diluted donkey anti-rabbit IgG Antibody conjugated to HRP (clone Poly4064) for competitor’s antibody (upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower). Lane M: MW ladder, M* indicates longer exposure.
  • W16155A_Pure_Cytokeratin7_2_ICC_Antibody_081717
    HaCaT cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:250 diluted (2 µg/ml) anti-KRT7 (clone W16155A) antibody overnight at 4 degrees followed by 1:250 diluted (2 µg/ml) Alexa Fluor® 594 (red) conjugated goat anti-rat IgG (clone Poly4054) antibody for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • W16155A_Pure_Cytokeratin7_4_ICC_Antibody_081717
    HaCaT cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:250 diluted (2 μg/ml) rat IgG2b, κ isotype control (clone RTK4530) overnight at 4 degrees followed by 1:250 diluted (2 μg/ml) Alexa Fluor® 594 (red) conjugated goat anti-rat IgG (clone Poly4054) antibody for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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601601 25 µg $101.00
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601602 100 µg $253.00
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Description

Keratin 7 (KRT7) belongs to type II keratin and is expressed in the epithelia lining the cavities of internal organs, gland ducts and blood vessels. The function of KRT7 is still not clear but it has been suggested involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). In cancer, the expression of KRT7 is observed in many types of carcinoma and is associated with neoplasm progression. The diverse and unique expression pattern of KRT7 is often used as a diagnosis indicator in epithelial origin carcinoma. For example, aberrant KRT7 expression is observed in triple negative breast cancer. In bladder cancer, loss of KRT7 results in promoting proliferation of the bladder urothelium.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human KRT7 peptide (446-462) conjugated to KLH.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml (1:250 - 1:2500 dilution). For immunocytochemistry, the recommended usage is 0.2 - 2.0 µg per mL (1:250 - 1:2500 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody does not react with mouse (in-house tested). Additional reported applications (for the relevant formats) include: spatial biology (IBEX)1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Peterson HM, et al. 2022. Adv Sci (Weinh). 9:e2200415. PubMed
  2. Zhang Y, et al. 2021. Front Oncol. 11:668707. PubMed
RRID
AB_2715904 (BioLegend Cat. No. 601601)
AB_2715904 (BioLegend Cat. No. 601602)

Antigen Details

Structure
Type II cytokeratin. 469 amino acids with a predicted molecular weight of 51 kD.
Distribution

Cytoplasm.

Function
KRT7 is a type II keratin, involved in the translational regulation of the human papillomavirus type 16 E7 mRNA.
Interaction
KRT7 interacts with EIF3S10, HPV16 E7, and GPER1.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References

1. Tajima Y, et al. 2017. Sci Rep. 7:40684.
2. Huang B, et al. 2016. Oncogene. 35:4927.
3. Szponar A, et al. 2012. Virchows Arch. 460:423.
4. Sano M, et al. 2010. Int J Oncol. 36:321.

Gene ID
3855 View all products for this Gene ID
UniProt
View information about Cytokeratin 7 on UniProt.org
Go To Top Version: 1    Revision Date: 08/17/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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