- Clone
- W19278B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Dickkopf-related protein 1, DKK1, Dickkopf WNT signaling pathway inhibitor 1
- Isotype
- Mouse IgG2b, κ
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Direct ELISA showing purified anti-human DKK-1 (clone W19278B) bound to plate-immobilized recombinant human DKK-1 protein (Cat. Nos. 778602, 778604, and 778606). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP goat anti-mouse IgG (Cat. No. 405306). TMB substrate (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 616552 | 100 µg | $265.00 | |||
DKK-1 (Dickkopf related protein 1) is a member of the Dickkopf family of proteins that include DKK-1, -2, -3, -4, and Soggy. DKK-1 was discovered by its ability to block Wnt signaling during early Xenopus embryogenesis. DKK-1 antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt proteins. DKK-1 forms a ternary complex with LRP5/6 and the transmembrane protein Kremen1/2, promoting internalization of LRP5/6 and down regulation of Wnt signaling. DKK-1 contains two conserved cysteine-rich domains separated by a linker region. The N-terminal cysteine-rich domain is unique to DKKs while the C-terminal cysteine-rich domain has a pattern of 10 cysteines similar to colipase. DKK-1 binds with LRP6 with high affinity and this interaction is enhanced by Kremens. The interactions between DKK-1 and both LRP6 and Kremens are mediated via C-terminal cysteine-rich domain. DKKs are expressed throughout development and play an important role by locally inhibiting Wnt regulated processes such as limb development, somitogenesis, anteroposterior axial patterning, and eye formation. In adults, DKKs are implicated in bone formation and bone disease, cancer and Alzheimer's disease. The balance between Wnt signaling and DKK-1 inhibition is important for bone homeostasis. Increased expression of DKK-1 are found in lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers. DKK-1 expression is decreased in malignant melanoma and colorectal cancers.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Recombinant DKK-1 Protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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Direct ELISA - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.
- Additional Product Notes
-
This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone W19278B) could function as a capture antibody when paired with Biotin anti-human DKK-1 antibody (Cat. No. 617053) (clone W19277H) as the detection antibody and using Recombinant Human DKK-1 protein (Cat. Nos. 778602, 778604, and 778606) as the assay standard.
- RRID
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AB_3083215 (BioLegend Cat. No. 616552)
Antigen Details
- Distribution
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Placenta, prostate, colon, spleen, and platelets.
- Function
- Wnt antagonist implicated in bone formation and bone disease, cancer and Alzheimer's disease. Also promotes type 2 cell-mediated inflammation.
- Interaction
- Bone
- Ligand/Receptor
- LRP5/6, Kremen-1, and Kremen-2
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Cancer Biomarkers, Neuroinflammation, Neuroscience, Stem Cells
- Antigen References
-
- Fedi P, et al. 1999. J Biol Chem. 274:19465-72.
- Krupnik VE, et al. 1999. Gene. 238:301-13.
- Mao B, et al. 2001. Nature. 411:321-5.
- Mukhopadhyay M, et al. 2001. Dev Cell. 1:423-34.
- Tian E, et al. 2003. New Eng J Med. 349:2483-94.
- Niehrs C. 2006. Oncogene. 25:7469-81.
- Diarra D, et al. 2007. Nature Med. 12:156-163.
- Fujita K and Janz S. 2007. Molecular Cancer. 6:71.
- Ahn VE, et al. 2011. Dev Cell. 21:862-73.
- Gene ID
- 22943 View all products for this Gene ID
- UniProt
- View information about DKK-1 on UniProt.org
Other Formats
View All DKK-1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human DKK-1 | W19278B | Direct ELISA |
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Purified anti-human DKK-1
Direct ELISA showing purified anti-human DKK-1 (clone W19278...