Purified anti-human FOXP3 Antibody

Pricing & Availability
Clone
206D (See other available formats)
Regulatory Status
RUO
Other Names
Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
Isotype
Mouse IgG1, κ
206D_110105
Cell extract from HEK293T cells transfected with human FoxP3 cDNA was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-FoxP3 antibody (clone 206D). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
  • 206D_110105
    Cell extract from HEK293T cells transfected with human FoxP3 cDNA was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-FoxP3 antibody (clone 206D). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
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320101 25 µg $129.00
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320102 100 µg $265.00
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Description

FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The 206D antibody recognizes human FOXP3 epitope in the region of amino acids 105-235.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Cynomolgus, Rhesus, Pigtailed Macaque
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length FOXP3 protein
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC, IHC-F, IHC-P - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. For Western blotting, the suggested working dilution(s) is ≤ 5.0 µg/ml in antibody dilution buffer. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1 and formalin-fixed paraffin-embedded sections1,8,19-20, and Western blotting1. The binding of 206D to FOXP3 can be partially blocked by 259D, but 206D does not show significant blocking effect on 259D binding.

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Roncador G, et al. 2005. Eur. J. Immunol. 35:1681.(IHC)
  2. Yang ZZ, et al. 2006. Blood 107:3639.
  3. Liu W, et al. 2006. J. Exp. Med. 203:1701.PubMed
  4. Bollyky PL, et al. 2007. J. Immunol. 179:744.
  5. Bell MP, et al. 2007. J. Immunol. 179:1893.
  6. Tran DQ, et al. 2007. Blood doi:10.1182/blood-2007-06-094656. PubMed
  7. Gao Q,et al.2007.J Clin Oncol.25:2586.(IHC) PubMed
  8. Pillai V,et al. 2008. Blood 111:463. PubMed
  9. Zheng Y, et al. 2008. J. Immunol. 181:1683. PubMed
  10. Zonios DI, et al. 2008.Blood112:287. PubMed
  11. Kavanagh B, et al. 2008. Blood. PubMed
  12. Nevala WK, et al. 2009. Clin Cancer Res. 15:1931. PubMed
  13. Grant J, et al. 2009. Cytometry B Clin Cytom. 76:69. PubMed
  14. Nigam P, et al. 2010. J. Immunol. 184:1690. PubMed
  15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (ICFC) PubMed
  16. Hartigan-O'Connor DJ,et al.2007.J Exp Med.204:2679. PubMed
  17. Raghaven S, et al. 2009. Ann Rheum Dis. 68:1908. PubMed
  18. Hodi FS, et al. 2014. Cancer Immunol Res. 2:632.(IHC) PubMed
  19. Sziros E, et al. 2015. Clin Cancer Res. 21:2840.(IHC) PubMed
Product Citations
  1. Martini R, et al. 2022. Cancer Discov. 12:2530. PubMed
  2. Gregory S, et al. 2023. Am J Cancer Res. 12:5564. PubMed
  3. Chen S, et al. 2020. Transl Lung Cancer Res. 1.404861111. PubMed
  4. Kavanagh B, et al. 2008. Blood. 112:1175. PubMed
  5. Richardson LG, et al. 2020. Oncoimmunology. 9:1806662. PubMed
  6. Riquelme E et al. 2019. Cell. 178(4):795-806 . PubMed
  7. Parra E,et al. 2017. Sci Rep.. 10.1038/s41598-017-13942-8. PubMed
  8. Chen P, et al. 2021. J Immunother Cancer. 9:. PubMed
  9. Haymaker C, et al. 2021. Cancer Discov. 11:1996. PubMed
  10. Nugent JL, et al. 2021. iScience. 24:103421. PubMed
  11. Karandikar V 2008. Blood. 111:463. PubMed
  12. Werneburg S, et al. 2020. Immunity. 52(1):167-182.e7.. PubMed
  13. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  14. Mitra A, et al. 2020. Nat Commun. 11:1839. PubMed
  15. Li Y, et al. 2019. Front Immunol. 0.460416667. PubMed
  16. Vence L, et al. 2019. Clin Cancer Res. 25:6501. PubMed
  17. Liu X, et al. 2022. STAR Protoc. 3:101310. PubMed
  18. Burlingame EA, et al. 2021. Cell Rep Methods. 1:. PubMed
  19. Boucher Y, et al. 2021. NPJ Precis Oncol. 5:62. PubMed
  20. Derks S, et al. 2020. Ann Oncol. 31:1011. PubMed
  21. de Groot J, et al. 2020. Neuro Oncol. 539:22. PubMed
  22. Helmink BA, et al. 2020. Nature. 577:549. PubMed
  23. Li W, et al. 2018. BMC Cancer. 1.589583333. PubMed
  24. Xie S, et al. 2020. Ann Transl Med. 1.384027778. PubMed
  25. Gao Q, et al. 2007. J Clinical Oncololgy. 25:2586. PubMed
  26. Zeigler-Johnson C, et al. 2016. PLoS One. 11: 0159109. PubMed
  27. Donnelly C, et al. 2018. Sci Rep. 0.625. PubMed
  28. Msaouel P, et al. 2020. Cancer Cell. 720:37. PubMed
  29. Wang H, et al. 2021. Onco Targets Ther. 14:2953. PubMed
  30. Hodi F, et al. 2014. Cancer Immunol Res. 2:632. PubMed
  31. Zsiros E, et al. 2015. Clin Cancer Res. 21:2840. PubMed
  32. Sadigh S, et al. 2020. Am J Clin Pathol. 387:153. PubMed
  33. Amaria RN, et al. 2018. Nat Med. 24:1649. PubMed
  34. Blessin NC, et al. 2019. Dis Markers. 2019:5160565. PubMed
  35. Durgin JS, et al. 2021. Front Oncol. 11:669071. PubMed
  36. Jiang M, et al. 2021. J Immunother Cancer. 9:. PubMed
  37. Luoma AM, et al. 2020. Cell. 182(3):655-671.e22. PubMed
  38. Zonios D, et al. 2008. Blood. 112:287. PubMed
RRID
AB_430880 (BioLegend Cat. No. 320101)
AB_430880 (BioLegend Cat. No. 320102)

Antigen Details

Structure
Forkhead/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains
Distribution

Nuclear; expressed in T regulatory cells

Function
Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis
Interaction
Interacts with DNA
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Hori S, et al. 2003. Science 299:1057.
2. Gandhi R, et al. 2010. Nat. Immunol. 11:846.

Regulation
FOXP3 is present at high levels in T regulatory cells, it can also be induced by T cell activation.
Gene ID
50943 View all products for this Gene ID
UniProt
View information about FOXP3 on UniProt.org
Go To Top Version: 5    Revision Date: 08/16/2022

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