- Clone
- A15080A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
- Isotype
- Mouse IgG2a, κ
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Total cell lysates (15 µg protein) from 293E untransfected (lane 1), 293E transfected with human FOXP3 (lane 2), and 293E transfected with human FOXP3 Delta2 (lane 3) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 0.1 µg/mL purified anti-FOXP3 Delta 2 (exon 2 deleted) antibody (clone A15080A). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 694802 | 100 µg | $265.00 | |||
FOXP3 is a transcription factor which is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 delta 2) lacking exon 2. This antibody reacts to human FOXP3 delta 2 (exon 2 deleted) form and weakly reacted to the full-length FOXP3.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human FOXP3 delta2 (exon 2 deleted) peptide conjugated to KLH.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is against human FOXP3 delta 2, molecular weight of 43 kD, and weakly reacts with full length human FOXP3, molecular weight 47 kD.
- RRID
-
AB_2650734 (BioLegend Cat. No. 694802)
Antigen Details
- Structure
- Forkhead/winged-helix transcription factor family, exon 2 deleted, approximately 43 kD, contains zinc finger and forkhead domains
- Distribution
-
Nuclear; expressed in T regulatory cells.
- Function
- Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis.
- Interaction
- IKZF3, STUB1, HSPA8 and HSPA1A/B, PPP1CA, PPP1CB, PPP1CG, KAT5, HDAC7, HDAC9, USP7, RUNX1, RORC, RUNX2, RUNX3 and IKZF4.
- Cell Type
- Tregs
- Biology Area
- Cell Biology, Cell Proliferation and Viability, Chromatin Remodeling/Epigenetics, Immunology, Innate Immunity, Signal Transduction, Transcription Factors, Ubiquitin/Protein Degradation
- Antigen References
-
1. Kristensen B, et al. 2015. Clin. Exp. Immunol. 180:58.
2. Roncador G, et al. 2005. Eur. J. Immunol. 35:1681.
3. Mayack S, et al. 2006. J. Immunol. 176:2059.
4. Yang ZZ, et al. 2006. Blood 107:3639.
5. Gavin MA, et al. 2006. Proc. Natl. Acad. Sci. USA 103:6659.
6. Groh V, et al. 2006. Nature Immunology 7:755.
7. Lewkowicz P, et al. 2006. J. Immunol. 177:7155.
8. Luke PP, et al. 2006. Am. J. Transplant. 6(9):2023.
9. Bamias G, et al. 2007. J. Immunol. 178:1809.
10. Valencia X, et al. 2007. J. Immunol. 178:2579.
11. Davidson TS, et al. 2007. J. Immunol. 178:4022. - Gene ID
- 50943 View all products for this Gene ID
- UniProt
- View information about FOXP3 on UniProt.org
Other Formats
View All FOXP3 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human FOXP3 Delta 2 (exon 2 deleted) | A15080A | WB |
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Purified anti-human FOXP3 Delta 2 (exon 2 deleted)
Total cell lysates (15 µg protein) from 293E untransfected (...