- Clone
- QA18A03 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
- Isotype
- Mouse IgG1, κ
-
Human peripheral blood lymphocytes were surface stained with CD4 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then intracellularly stained with purified anti-human FOXP3 recombinant (clone QA18A03) (left) or purified mouse IgG1, κ isotype control (right), followed by anti-mouse IgG PE secondary antibody.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 364702 | 100 µg | $265.00 | |||
FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2892440 (BioLegend Cat. No. 364702)
Antigen Details
- Structure
- Forkhead/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains
- Distribution
-
Nuclear; expressed in T regulatory cells
- Function
- Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis
- Interaction
- Interacts with DNA
- Antigen References
-
1. Hori S, et al. 2003. Science 299:1057.
2. Gandhi R, et al. 2010. Nat. Immunol. 11:846. - Gene ID
- 50943 View all products for this Gene ID
- UniProt
- View information about FOXP3 on UniProt.org
Other Formats
View All FOXP3 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-human FOXP3 Recombinant Antibody | QA18A03 | ICFC |
| Purified anti-human FOXP3 Recombinant Antibody | QA18A03 | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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PE anti-human FOXP3 Recombinant Antibody
Human peripheral blood lymphocytes were surface stained with... -
Purified anti-human FOXP3 Recombinant Antibody
Human peripheral blood lymphocytes were surface stained with...