- Clone
- BVD13-3A5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Granulocyte-Colony Stimulating Factor, CSF-β, Pluripoietin, Granulocytic neutrophil colony stimulating activity (G-CSA), Macrophage-granulocyte inducer-2 (MGI-2)
- Isotype
- Rat IgG1, κ
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 502101 | 50 µg | $94.00 | |||
| 502102 | 500 µg | $235.00 | |||
Granulocyte - colony stimulating factor (G-CSF) is a potent stimulator of bone marrow cells especially those of neutrophilic granulocyte lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils. G-CSF is produced primarily by monocytes and macrophages upon activation by endotoxin, TNF-alpha or IFN-gamma.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- E. coli-expressed, recombinant human G-CSF
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture - Quality tested
IHC - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA or ELISPOT Capture1,2: The purified BVD13-3A5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD11-37G10 antibody (Cat. No. 506702) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.
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Application References
(PubMed link indicates BioLegend citation) -
- Abrams J. 1995. Curr. Prot. Immunol.. John Wiley and Sons New York. Unit 6.20.
- Abrams J, et al. 1992. Immunol. Rev. 127:5.
- Andersson J, et al. 1994. Immunology 83:16.
- Sander B, et al. 1991. Immunol. Rev. 119:65.
- Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
- Product Citations
-
- RRID
-
AB_2083733 (BioLegend Cat. No. 502101)
AB_2083733 (BioLegend Cat. No. 502102)
Antigen Details
- Structure
- Cytokine; 21 kD (Mammalian)
- Bioactivity
- Growth/differentiation/activating factor for neutrophils; proliferation/migration endothelial cells cells; synergistic with IL-3 growth of hematopoietic progenitors
- Cell Sources
- Macrophages, monocytes, neutrophils, fibroblasts, endothelial cells, bone marrow stromal cells
- Cell Targets
- Hematopoietic progenitors of neutrophil/granulocyte lineage, endothelial cells
- Receptors
- CD114 (G-CSFR)
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Demetri G, et al. 1991. Blood 78:2791.
3. Moore M. 1991. Annu. Rev. Immunol. 9:159.
4. Negrin R, et al. 1992. Adv. Pharmacol. 23:263. - Regulation
- Upregulated by IL-1, IL-17, TNF-α, IFN-γ, GM-CSF; downregulated by prostaglandin E2
- Gene ID
- 1441 View all products for this Gene ID
- UniProt
- View information about G-CSF on UniProt.org
Other Formats
View All G-CSF Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human G-CSF | BVD13-3A5 | ELISA Capture,IHC |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-human G-CSF
