- Clone
- W21101F (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Lgals9, Gal-9, Ecalectin, Tumor antigen HOM-HD-21, Ecalectin
- Isotype
- Mouse IgG1, κ
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Human peripheral blood lymphocytes were stimulated with Cell Activation Cocktail (without Brefeldin A) (Cat. No. 423301) for 4 hours and surface stained with anti-human CD3 (clone UCHT1) APC. Cells were then fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803), and intracellularly stained with purified anti-human/mouse Galectin-9 (clone W21101F) (left) or purified mouse IgG1, κ isotype control (right), followed by goat anti-mouse IgG PE. -
MOLT-4 (human T-lymphoblastic leukemia cell line) cells were fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803), then intracellularly stained with purified anti-human/mouse Galectin-9 (clone W21101F) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by goat anti-mouse IgG PE. -
C57BL/6 mouse thymocytes were fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803), then intracellularly stained with purified anti-human/mouse Galectin-9 (clone W21101F) (left) or purified mouse IgG1, κ isotype control (right), followed by goat anti-mouse IgG PE. Cells were then co-stained with anti-mouse CD4 (clone GK1.5) APC. -
THP-1 cells, a human monocyte cell line isolated from peripheral blood of an acute monocytic leukemia patient, were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) and permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained without (panel A) and with (panel B) 5 μg/mL of the purified anti-human/mouse Galectin-9 antibody overnight at 4°C followed by goat anti-mouse IgG (clone Poly4053) Alexa Fluor® 647 for an hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 μm
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 390804 | 100 µg | $230.00 | |||
Galectin-9 is a mammalian lectin with a molecular weight around 50 kD. It is a member of the β-galactoside-binding family. With two conserved carbohydrate recognition domains (CRDs), galectin-9 binds small β-galactosides as well as complex glycoconjugates. HAVCR2/TIM3 has been reported as one of its ligands. Galectin-9 may be retained intracellularly or transported to the cell surface where it can be cleaved to generate a soluble form. Galectin-9 is expressed by lymphocytes, dendritic cells, granulocytes, eosinophils, astrocytes, endothelial cells, fibroblasts, and thymus epithelial cells. It can be induced by cytokines in various cell types and is involved in cell aggregation, adhesion, chemotaxis, and apoptosis. Galectin-9 induces regulatory T cells and suppresses Th1 and Th17 responses.
Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human galectin-9 protein (a.a 2-323)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ICFC - Quality tested
FC, ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration of 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance in each application.
- Application Notes
-
Clone W21101F does not block the staining of reference clone 9M1-3.
Recombinant protein completely blocks the staining of test clone W21101F. - RRID
-
AB_3662367 (BioLegend Cat. No. 390804)
Antigen Details
- Structure
- Member of the β-galactoside-binding lectin family. Molecular weight around 50 kD. Binds small β-galactosides as well as complex glycoconjugates with two conserved carbohydrate recognition domains (CRDs).
- Distribution
-
Lymphocytes, dendritic cells, neutrophils, eosinophils, astrocytes, endothelial cells, fibroblasts, thymus stromal/epithelial cells
- Function
- Cell aggregation, adhesion, chemotaxis, apoptosis, suppression of Th1 and Th17 responses, and induction of regulatory T cells
- Interaction
- Astrocytes, dendritic cells, endothelial cells, eosinophils, epithelial cells, fibroblasts, lymphocytes, neutrophils, tregs
- Ligand/Receptor
- HAVCR2/TIM3
- Cell Type
- Astrocytes, Dendritic cells, Endothelial cells, Eosinophils, Epithelial cells, Fibroblasts, Lymphocytes, Neutrophils, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
- Molecular Family
- Adhesion Molecules, Immune Checkpoint Receptors
- Antigen References
-
- Seki M, et al. 2008. Clin Immunol. 127:78-88.
- Tsuboi Y, et al. 2007. Clin Immunol. 124:221-33.
- Zhu C, et al. 2005. Nat Immunol. 6:1245-52.
- Dunphy JL, et al. 2002. J Biol Chem. 277:14916-24.
- Gene ID
- 8795 View all products for this Gene ID
- UniProt
- View information about Galectin-9 on UniProt.org
Other Formats
View All Galectin-9 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human/mouse Galectin-9 | W21101F | ICFC,FC,ICC |
| PE anti-human/mouse Galectin-9 | W21101F | ICFC,FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-human/mouse Galectin-9
Human peripheral blood lymphocytes were stimulated with Cell... 
MOLT-4 (human T-lymphoblastic leukemia cell line) cells were... 
C57BL/6 mouse thymocytes were fixed and permeabilized using ... 
THP-1 cells, a human monocyte cell line isolated from periph... -
PE anti-human/mouse Galectin-9
Human peripheral blood lymphocytes were stimulated with Cell... 
MOLT-4 (human T-lymphoblastic leukemia cell line) cells were... 
C57BL/6 mouse splenocytes were surface stained with anti-mou...