Purified anti-human GM-CSF (Maxpar® Ready) Antibody

Pricing & Availability
Clone
BVD2-21C11 (See other available formats)
Regulatory Status
RUO
Other Names
Granulocyte/macrophage colony-stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA)
Isotype
Rat IgG2a, κ
1_BVD2-21C11_Purified_GM-CSF_Cytof_1_061014.jpg
Human PBMCs were incubated for 6 hours in media alone (top) or with PMA and Ionomycin (bottom) in the presence of monensin and brefeldin A. Cells were then fixed, permeabilized, and stained with 154Sm-anti-CD45 (HI30) and 159Tb-anti-GM-CSF (BVD2-21C11). Data provided by DVS Sciences.
  • 1_BVD2-21C11_Purified_GM-CSF_Cytof_1_061014.jpg
    Human PBMCs were incubated for 6 hours in media alone (top) or with PMA and Ionomycin (bottom) in the presence of monensin and brefeldin A. Cells were then fixed, permeabilized, and stained with 154Sm-anti-CD45 (HI30) and 159Tb-anti-GM-CSF (BVD2-21C11). Data provided by DVS Sciences.
  • 2_BVD2-21C11_Purified_GM-CSF_Cytof_2_061014.jpg
  • 3_BVD2-21C11_PURE_GM-CSF_ELISA_092117.png
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Cat # Size Price Quantity Check Availability
502315 100 µg $171.00
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Description

Granulocyte/macrophage - colony stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T cells, B cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli-expressed, recombinant human GM-CSF.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
CyTOF® - Verified
Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

Additional reported applications (for the relevant formats) include: ELISA1-4 or ELISPOT3,4 Detection, Neutralization1, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated5,6 and acetone-fixed7 frozen tissue sections, and immunocytochemistry.

Note: For testing human GM-CSF in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 432001 to 432006) are specially developed and recommended.

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA, Neut, IP)
  2. Abrams J, et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker New York. p.133. (ELISA)
  3. Bacchetta R, et al. 1990. J. Immunol. 144:902. (ELISA)
  4. Kita H, et al. 1991. J. Exp. Med. 174:745. (ELISA)
  5. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. (IHC)
  6. Andersson J, et al. 1994. Immunology 83:16. (IHC)
  7. Rasouli J, et al. 2015. J. Immunol. 11:5085-93. (IHC)
RRID
AB_2563770 (BioLegend Cat. No. 502315)

Antigen Details

Structure
Cytokine; 22 kD (Mammalian)
Bioactivity
Growth/development factor for granulocyte/macrophage progenitors; differentiates myeloblasts/monoblasts; synergizes with Epo proliferation of erythroid/megakaryocytic progenitors
Cell Sources
T cells, macrophages, fibroblasts, endothelial cells, mast cells
Cell Targets
Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts
Receptors
Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131), which is also shared with the IL-3 and IL-5 receptor α chains
Biology Area
Cell Biology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Demetri G, et al. 1991. Blood 78:2791.
3. Fan D, et al. 1991. In vivo 5:571.
4. Negrin R, et al. 1992. Adv. Pharmacol. 23:263.

Regulation
Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans
Gene ID
1437 View all products for this Gene ID
UniProt
View information about GM-CSF on UniProt.org

Other Formats

View All GM-CSF Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-human GM-CSF BVD2-21C11 ELISA Detection,ELISPOT Detection,ICFC
PE anti-human GM-CSF BVD2-21C11 ICFC
Purified anti-human GM-CSF BVD2-21C11 ELISA Capture,ICFC,IP,WB
PerCP/Cyanine5.5 anti-human GM-CSF BVD2-21C11 ICFC
APC anti-human GM-CSF BVD2-21C11 ICFC
Pacific Blue™ anti-human GM-CSF BVD2-21C11 ICFC
Purified anti-human GM-CSF (Maxpar® Ready) BVD2-21C11 ELISA Capture,CyTOF®
PE/Dazzle™ 594 anti-human GM-CSF BVD2-21C11 ICFC
Ultra-LEAF™ Purified anti-human GM-CSF BVD2-21C11 Neut
TotalSeq™-B0943 anti-human GM-CSF BVD2-21C11 ICPG
Brilliant Violet 421™ anti-human GM-CSF BVD2-21C11 ICFC
Go To Top Version: 2    Revision Date: 07/22/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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