- Clone
- W19372J (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Scatter Factor (SF), Hepatopoietin (HPTA), Lung fibroblast-derived mitogen
- Isotype
- Mouse IgG2a, κ
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Direct ELISA showing purified anti-human HGF (clone W19372J) bound to plate-immobilized recombinant human HGF protein (Cat. No. 596402). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Goat anti-mouse IgG (Cat. No. 405306). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 613052 | 100 µg | $235.00 | |||
HGF is a dimeric molecule composed of the α-subunit and β-subunit, linked by a disulfide bond. The C-terminus of the α-subunit is followed directly by the N-terminus of the β-subunit. HGF protects the epithelium, neurons, and cardiomyocytes during organ diseases. This protective effect is mediated through anti-apoptotic signals via inhibition of caspase-3 activity or induction of anti-apoptotic molecules, such as Bcl-xL. In addition, HGF prohibits Fas-mediated apoptosis signals via sequestration of Fas and Met receptor on cell surfaces. Also, HGF elicits the regression of fibrosis in numerous organs, such as scleroderma, cardiomyopathy, vocal scarring, and peritoneal fibrosis.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Recombinant HGF Protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
Direct ELISA - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.
- Additional Product Notes
-
This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone W19372J) could function as a capture antibody when paired with Biotin anti-human HGF antibody (Cat. No. 613153) (clone W19371A) as the detection antibody and using Recombinant Human HGF protein (Cat. No. 596401 or Cat. No. 596402) as the assay standard.
- RRID
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AB_2941610 (BioLegend Cat. No. 613052)
Antigen Details
- Structure
- Heterodimer (alpha and beta chains)
- Distribution
-
Mesenchymal cells, liver sinusoidal endothelial, macrophages
- Function
- Stimulates epithelial cell proliferation, motility, morphogenesis, and angiogenesis. Hypoxia and hyperglycemia suppress HGF production. In addition, TGF-β and angiotensin-II depress HGF production in vitro.
- Interaction
- Hepathocytes, epithelial cells
- Ligand/Receptor
- MET receptor
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Angiogenesis, Cell Biology, Stem Cells
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
- Kataoka H, et al. 2000. J Biol Chem. 275:40453-62.
- Wang X, et al. 2002. Mol Cell. 9:411-21.
- Wu MH, et al. 2004. Gene Ther. 11:170-80.
- Mizuno S, et al. 2008. Front Biosci. 13:7072-86.
- D'Angelo F, et al. 2013. Clin Exp Immunol. 174:60-72.
- Gene ID
- 3082 View all products for this Gene ID
- UniProt
- View information about HGF on UniProt.org
Other Formats
View All HGF Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human HGF | W19372J | Direct ELISA |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-human HGF
Direct ELISA showing purified anti-human HGF (clone W19372J)...