- Clone
- W20086C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interferon-β, Fibroblast interferon (Fi-IFN, F-IFN), Type-1 interferon, acid-stable interferon, R1-GI factor, IFN-b, IFN-beta
- Isotype
- Mouse IgG2a, κ
-
Direct ELISA showing purified anti-human IFN-β (clone W20086C) bound to plate-immobilized recombinant human IFN-β protein. The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP goat anti-mouse IgG (Cat. No. 405306). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 631952 | 100 µg | $420.00 | |||
The IFN-β/W20086C clone recognizes natural human IFN-β and recombinant β1. It is capable of neutralizing IFN-β.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Recombinant IFN-β Protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
Direct ELISA - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.
- Additional Product Notes
-
This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone W20086C) could function as a capture antibody when paired with biotin anti-human IFN-β antibody (Cat. No. 617553) (clone W20086B) as the detection antibody and using Recombinant human IFN-β protein as the assay standard.
- RRID
-
AB_3106026 (BioLegend Cat. No. 631952)
Antigen Details
- Structure
- Interferon; contains 187 amino acids with predicted molecular weight 22 kD.
- Distribution
-
Fibroblasts, epithelial cells
- Interaction
- Broad, includes NK cells, T suppressor cells, activated monocytes
- Ligand/Receptor
- IFNα/βR (IFNAR-1, IFNAR-2)
- Biology Area
- Cell Biology, Signal Transduction
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
- Nagarajan U. 2011. Crit Rev Immunol. 31:459-74.
- Honda K, et al. 2005. Int Immunol. 17:1367.
- Gene ID
- 3456 View all products for this Gene ID
- UniProt
- View information about IFN-beta on UniProt.org
Other Formats
View All IFN-β Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human IFN-β | W20086C | Direct ELISA |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human IFN-β
Direct ELISA showing purified anti-human IFN-β (clone W20086...