- Clone
- JK1RA-1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-1 receptor antagonist, IL-1 inhibitor, IL-1 receptor antagonist protein (IRAP), IL-1 inhibitory activity, ILS, MG50, IL-1RN
- Isotype
- Mouse IgG1
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 509901 | 50 µg | $106.00 | |||
| 509902 | 500 µg | $347.00 | |||
Interleukin-1 receptor antagonist (IL-1RA) is composed of three isoforms, one secreted (sIL-1Rα/IL-1Rα1), two intracellular (IL-1Rα2/IL-1Rα3) and produced by monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma cells, keratinocytes, and chondrocytes. It is upregulated by IL-1β, IL-4, IL-6 and downregulated by glucocorticoids. The JK1RA-1 antibody reacts with human interleukin-1 receptor antagonist (IL-1RA).
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human IL-1 receptor antagonist
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.05% sodium azide, 0.02% Proclin 950.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture
- Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 4-6 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA Capture: The purified JK1RA-1 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5095 (Cat. No. 509501) antibody as the detecting antibody.
-
Application References
(PubMed link indicates BioLegend citation) -
- Xi X, et al. 2011. Invest Ophthalmol Vis Sci. 52:4859. PubMed.
- Product Citations
-
- RRID
-
AB_315547 (BioLegend Cat. No. 509901)
AB_315547 (BioLegend Cat. No. 509902)
Antigen Details
- Structure
- Cytokine; three isoforms, one secreted (sIL-1Rα/IL-1Rα1), two intracellular (IL-1Rα2/IL-1Rα3); 17 kD (Mammalian)
- Bioactivity
- Storage forms that are released upon cell death to limit inflammation caused by cell debris; competitive inhibitor of IL-1 receptor binding; antagonizes IL-1 activities without having agonist effects; does not initiate intracellular signal transduction pr
- Cell Sources
- Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma cells, keratino
- Cell Targets
- B cells, T cells, monocytes
- Receptors
- Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Carter D, et al. 1990. Nature 344:633.
2. Chirivi R, et al. 1993. Cancer Res. 53:5051.
3. Holtkamp G, et al. 1999. Eur. J. Immunol. 29:215.
4. Kim J, et al. 2003. Gene Ther. 10:1543. - Regulation
- Upregulated by IL-1β, IL-4, IL-6; downregulated by glucocorticoids
- Gene ID
- 3557 View all products for this Gene ID
- UniProt
- View information about IL-1RA on UniProt.org
Other Formats
View All IL-1RA Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human IL-1 RA | JK1RA-1 | ELISA Capture |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-human IL-1 RA
