- Clone
- JES10-5A2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-13, NC30
- Isotype
- Rat IgG1, κ
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 501901 | 50 µg | $106.00 | |||
| 501902 | 500 µg | $252.00 | |||
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 lymphocytes. IL-13 shares 30% amino acid sequence homology with IL-4 and demonstrates similar biological activities. The biological activities of IL-13 include: suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, and suppression of proinflammatory cytokine secretion. The JES10-5A2 antibody reacts with human interleukin-13 (IL-13). The JES10-5A2 antibody can neutralize the bioactivity of natural or recombinant IL-13.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- COS-expressed, recombinant human IL-13
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture - Quality tested
IHC, WB - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For ELISA applications, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-13 recombinant protein ranging from 2000 to 8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA or ELISPOT Capture1,2: The Ultra-LEAF™ Purified JES10-5A2 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5020 antibody (Cat. No. 502001) as the detecting antibody. The Ultra-LEAF™ Purified antibody is suggested for ELISPOT capture.
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Application References
(PubMed link indicates BioLegend citation) -
- McKenzie, A., et al. 1994. Curr. Prot. Immunol.. John Wiley & Sons. New York. Section 6.18.
- Abrams, J. 1995. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.20.
- Schaerli, P., et al. 2000. J. Exp. Med. 192:1553.
- Andersson, J., et al. 1994. Immunology 83:16.
- Skansen-Saphir, U., et al. 1994. Eur. J. Immunol. 24:916.
- Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
- Gelfand, E. W., et al. 2006. J. Aller. and Clinic. Immunol. 117:577.
- Knorr, C., et al. 1999. Am. J. Pathol. 155:2019.
- Product Citations
-
- RRID
-
AB_315196 (BioLegend Cat. No. 501901)
AB_315196 (BioLegend Cat. No. 501902)
Antigen Details
- Structure
- Cytokine; 17 kD (Mammalian)
- Bioactivity
- Suppression of macrophage cytotoxic activity; upregulation of IL-1RA expression; suppression of IL-1, IL-6, IL-8, IL-10, IL-12, chemokines MCP, MIP-1; differentiation of B cells, monocytes
- Cell Sources
- Activated T cells, mast cells, NK cells
- Cell Targets
- B cells, monocytes, macrophages, basophils
- Receptors
- Moderate affinity IL-13Rα1; high affinity heterodimer IL-13Rα1/IL-4Rα also binds IL-4; both receptor complexes have γ-subunit in common with IL-2R, IL-4R, IL-7R, IL-15R ; high affinity IL-13Rα2 incapable of signaling
- Cell Type
- Tregs
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hilton, D., et al. 1996. P. Natl. Acad. Sci. USA 93:497.
3. Obiri, N., et al. 1995. J. Biol. Chem. 270:8797.
4. Ochensberger, B., et al. 1996. Blood 88:3028. - Regulation
- IL-13Rα2 possible antagonist
- Gene ID
- 3596 View all products for this Gene ID
- UniProt
- View information about IL-13 on UniProt.org
Other Formats
View All IL-13 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| APC anti-human IL-13 | JES10-5A2 | ICFC |
| LEAF™ Purified anti-human IL-13 | JES10-5A2 | ELISA Capture, ELISPOT Capture, ICFC, IHC, Neut, WB |
| PE anti-human IL-13 | JES10-5A2 | ICFC |
| Purified anti-human IL-13 | JES10-5A2 | ELISA Capture,IHC,WB |
| PerCP/Cyanine5.5 anti-human IL-13 | JES10-5A2 | ICFC |
| PE/Cyanine7 anti-human IL-13 | JES10-5A2 | ICFC |
| Brilliant Violet 421™ anti-human IL-13 | JES10-5A2 | ICFC |
| Ultra-LEAF™ Purified anti-human IL-13 | JES10-5A2 | ELISA Capture,ELISPOT Capture,ICFC,IHC,Neut,WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human IL-13
Enriched human CD4+ T cells were stimulated with PMA+ionomyc... -
LEAF™ Purified anti-human IL-13
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PE anti-human IL-13
PMA+ionomycin-stimulated T lymphocytes intracellularly stain... -
Purified anti-human IL-13
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PerCP/Cyanine5.5 anti-human IL-13
PMA-restimulated Th2 polarized human peripheral blood lympho... -
PE/Cyanine7 anti-human IL-13
TH2 polarized human peripheral blood lymphocytes were surfac... -
Brilliant Violet 421™ anti-human IL-13
TH2 polarized human peripheral blood lymphocytes were surfac... 
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Ultra-LEAF™ Purified anti-human IL-13