Purified anti-human IL-17A Antibody

Pricing & Availability
Clone
BL168 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin IL-17A, IL-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
Isotype
Mouse IgG1, κ
IL-17A_Pure_021808.jpg
PMA (50 ng/ml) + ionomycin (1 µg/ml)-stimulated (6 hours + monensin, 2 µM) human peripheral blood lymphocytes intracellularly stained with anti-IFNγ (4S.B3) PE and anti-IL-17A (BL168) FITC
  • IL-17A_Pure_021808.jpg
    PMA (50 ng/ml) + ionomycin (1 µg/ml)-stimulated (6 hours + monensin, 2 µM) human peripheral blood lymphocytes intracellularly stained with anti-IFNγ (4S.B3) PE and anti-IL-17A (BL168) FITC
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Cat # Size Price Quantity Check Availability
512301 50 µg $89.00
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512302 500 µg $258.00
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Description

IL-17A is the founding member of the IL-17 family, a group of six structurally related pro-inflammatory cytokines. IL-17A, secreted by activated CD4+ Th17 cell subpopulation, elicits multiple biological activities on a variety of cells including: the induction of IL-6, IL-8, G-CSF, and PGE2 production in epithelial, endothelial or fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-κB and costimulation of T cell proliferation. Recent studies demonstrated that, in mice, activated IL-17-secreting CD4+ helper T cells (Th17 cells) mediate an autoimmune arthritis that clinically and immunologically resembles rheumatoid arthritis (RA). Human IL-17A shows 63%, 63%, and 72% amino acid sequence identity to rat IL-17A, mouse IL-17A, and a protein encoded by the ORF13 gene of herpesvirus Saimiri (HVS), respectively.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant full length human IL-17A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. It isrecommended that the reagent be titrated for optimalperformance for each application.

Product Citations
  1. Alroqi F, et al. 2017. J Clin Immunol. . 10.1007/s10875-017-0451-1. PubMed
  2. Matthias J, et al. 2020. J Clin Invest. 130:4587. PubMed
  3. Willing A, et al. 2018. J Immunol. 200:974. PubMed
  4. Kessel C, et al. 2017. Arthritis Rheumatol. 69:1480. PubMed
  5. Aravena O, et al. 2017. Arthritis Res Ther. 19:8. PubMed
  6. Ma F, et al. 2017. Exp Cell Res.. 10.1016/j.yexcr.2017.10.011. PubMed
  7. Sattler A, et al. 2021. Am J Transplant. 21:87. PubMed
  8. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  9. Lückel C, et al. 2019. Nat Commun. 4.390277778. PubMed
  10. Singh AK, et al. 2017. Front Immunol. 0.513888889. PubMed
  11. Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
  12. Schwabenland M, et al. 2021. Immunity. . PubMed
  13. Umeda M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
RRID
AB_961399 (BioLegend Cat. No. 512301)
AB_961399 (BioLegend Cat. No. 512302)

Antigen Details

Structure
Cytokine; dimer; ~15 kD (Mammalian)
Bioactivity
Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Cell Sources
Activated T cell subpopulation
Cell Targets
Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors
IL-17R
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Hirota K, et al. 2007. J. Exp. Med. 204:41.
2. Furuzawa-Carballeda J, et al. 2007. Autoimmun. Rev. 6:169.
3. Witowski J, et al. 2007. Kidney Int. 71:514.
4. Gaffen SL, et al. 2006. Vitam. Horm. 74:255.
5. Hymowitz S, et al. 2001. EMBO J. 20:5332.

Gene ID
3605 View all products for this Gene ID
UniProt
View information about IL-17A on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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