Purified anti-human IL-6 Antibody

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

COS-7- expressed, recombinant human IL-6

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

ELISA Capture - Quality tested
CyTOF® - Verified
ELISPOT Capture, Neut, ICFC - Reported in literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5 - 2.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-6 recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture^1-3,7: The purified MQ2-13A5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MQ2-39C3 antibody (Cat. No. 501202) as the detecting antibody.

Neutralization^1-3,5,6: The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-6 bioactivity (Cat. No. 501110). The MQ2-13A5 antibody can neutralize the bioactivity of natural or recombinant IL-6.

Additional applications (for the relevant formats) include: intracellular flow cytometry¹⁰.

Application References

(PubMed link indicates BioLegend citation)

1. Abrams J, et al. 1992. Immunol. Rev. 127:5.
2. Abrams JS. 2001. Curr. Protoc. Immunol. Unit 6.20.
3. Gaines Das R, et al. 1993. J. Immunol. Methods 160:147.
4. Enriquez J, et al. 2002. Adv. Perit Dial. 18:177.
5. Zou JP, et al. 1999. J. Immunol. 162:4882.
6. Wyant TL, et al. 1999. Infect. Immun. 67:1338.
7. Lesmeister MJ, et al. 2005. Reprod. Biol. Endocrinol. 3:74.
8. Terasaka Y, et al. 2010. Invest. Ophthalmol. Vis. Sci. 51:2441 PubMed
9. Girndt M, et al. 1998. J. Am. Soc. Nephrol. 9:1689.

Product Citations

1. Glass MC, et al. 2022. Cell Rep. 39:110728. PubMed
2. Terasaka Y, et al. 2010. Invest Ophthalmol Vis Sci. 51:2441. PubMed
3. Fosdick MG, et al. 2022. Sci Rep. 12:13506. PubMed
4. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
5. Amici SA, et al. 2018. Front Immunol. 16:e2005090. PubMed

RRID

AB_315150 (BioLegend Cat. No. 501101)
AB_315150 (BioLegend Cat. No. 501102)

Antigen Details

Structure

Neuropoietic cytokine family; 26 kD (Mammalian)

Bioactivity

Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction

Cell Sources

T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells

Cell Targets

T cells, B cells, hepatocytes, cholinergic neurons

Receptors

Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130)

Biology Area

Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience

Molecular Family

Cytokines/Chemokines

Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hirano T. 1998. Int. Rev. Immunol. 16:249.
3. Patterson P. 1992. Curr. Opin. Neurobiol. 2:94.
4. van Oers M, et al. 1993. Ann. Hematol. 66:219.

Regulation

Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, and IL-17; downregulated by glucocorticoids IL-4 and TGF-β

Gene ID

3569 View all products for this Gene ID

UniProt

View information about IL-6 on UniProt.org

Related Protocols

• Active Protocols: Sandwich ELISA - Video
• Sandwich ELISA Protocol

Other Formats