- Clone
- 359-238-8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Lymphotoxin-α (LT- α), Tumor necrosis factor-β (TNF-β), Coley's toxin, Hemorrhagic factor, Necrosin, Natural killer cytotoxic factor (NKCF), Differentiation inducing factor (DIF), TNFSF-1
- Isotype
- Mouse IgG1, κ
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 503002 | 500 µg | $235.00 | |||
Lymphotoxin-α (LT-α), also known as tumor necrosis factor-beta (TNF-β), is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. LT-α possesses a signal peptide sequence and is a secreted protein; however, LT-α is also present on the surface of activated T, B and LAK cells as a complex with LT-β. Bioactive LT-α exists as a homotrimer.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- E.coli expressed, recombinant human LT-α.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA or ELISPOT Capture1,2: The purified 359-238-8 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 359-81-11 antibody (Cat. No. 503104) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.
Neutralization1,2: The 359-238-8 antibody can neutralize the bioactivity of human LT-α. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human LT-α bioactivity (Cat. No. 503004). -
Application References
(PubMed link indicates BioLegend citation) -
- Meager A, et al. 1987. J. Immunol. Methods 104:31.
- Meager A, et al. 1987. Hybridoma. 6:305.
- Product Citations
-
- RRID
-
AB_315268 (BioLegend Cat. No. 503002)
Antigen Details
- Structure
- TNF superfamily; trimer; 25 kD (Mammalian)
- Bioactivity
- Transformed cell cytotoxicity; mediator of inflammatory and immune functions; fibroblast synthesis of GM-CSF, G-CSF, IL-1, collagenase, prostaglandin E2; monocyte terminal differentiation, synthesis of G-CSF; neutrophil chemoattractant, production of reac
- Cell Sources
- Activated T and B cells, fibroblasts, astrocytes, myeloma, endothelial cells, epithelial cells
- Cell Targets
- Monocytes, B cells, fibroblasts, neutrophils, osteoclasts, keratinocytes, endothelial cells
- Receptors
- TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Aggarwal B, et al. Eds. 1992. Tumor necrosis factors:structure, function, and mechanism of action. Marcel Dekker Inc.
3. Bonavida B, et al. Eds. 1990. Tumor necrosis factor:structure, mechanisms of action, role in disease and therapy. Karger, Basel.
4. Paul N, et al. 1987. Annu. Rev. Immunol. 6:407. - Regulation
- Type II integral membrane protein, forms heterotrimer with type II integral membrane protein LT-β either as LTα1β2 or LTα2β1; processed secreted form is trimeric
- Gene ID
- 4049 View all products for this Gene ID
- UniProt
- View information about LT-alpha on UniProt.org
Other Formats
View All LT-α Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human LT-α (TNF-β) | 359-238-8 | ELISA Capture |
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Purified anti-human LT-α (TNF-β)
