Purified anti-human LYPD3 Antibody

Pricing & Availability
Clone
11A1 (See other available formats)
Regulatory Status
RUO
Other Names
C4.4A, GPI-Anchored Metastasis-Associated Protein C4.4A Homolog, LY6/PLAUR Domain Containing 3
Isotype
Mouse IgG2b, κ
11A1_PURE_LYPD3_Antibody_1_022522
Human paraffin-embedded skin tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human LYPD3 (clone 11A1) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with goat anti-mouse (clone Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was captured with a 10X objective.
  • 11A1_PURE_LYPD3_Antibody_1_022522
    Human paraffin-embedded skin tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human LYPD3 (clone 11A1) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with goat anti-mouse (clone Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was captured with a 10X objective.
  • 11A1_PURE_LYPD3_Antibody_2_031022
    MCF-7 cells were stained with purified anti-human LYPD3 (clone 11A1) (filled histogram) or purified mouse IgG2b, κ isotype control (clone MPC-11) (open histogram) followed by PE anti-mouse IgG.
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Cat # Size Price Quantity Check Availability
364502 100 µg $364.00
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Description

C4.4A, also known as Ly6/PLAUR domain containing 3 (LYPD-3), is a GPI-linked protein with structural similarity to the urokinase-type plasminogen activator receptor (uPAR). C4.4A is also expressed in the placenta, skin and urothelium and can be found in suprabrasal layers of stratified squamous epithelium and is upregulated on migrating keratinocytes during wound healing. Human C4.4A mRNA has been detected in cancer cell lines, including melanoma, breast, bladder, and renal cell carcinoma, as well as in tumor tissue samples from malignant melanoma, colorectal cancer (CRC), breast cancer, lung carcinoma, and urothelial tumors. Expressed in placenta, skin and urothelium. Found in suprabasal keratinocytes of chronic wounds. Weak expression is found in esophagus and peripheral blood mononuclear cells.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested

FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2922576 (BioLegend Cat. No. 364502)

Antigen Details

Distribution

Placenta, skin, urothelium, keratinocytes

Function
Interacting selectively and non-covalently with laminins, glycoproteins that are major constituents of the basement membrane of cells
Interaction
LGALS3 (By similarity). AGR2 and AGR3
Ligand/Receptor
Laminin-1 and laminin-5
Biology Area
Cell Adhesion
Molecular Family
Adhesion Molecules
Antigen References
  1. Smith BA, et al. 2001. Cancer Res. 61:1678-85.
  2. Würfle J, et al. 2001. Gene. 262:35-41.
Gene ID
27076 View all products for this Gene ID
UniProt
View information about LYPD3 on UniProt.org
Go To Top Version: 1    Revision Date: 02/25/2022

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