- Clone
- L1617F1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CSF1, CSF-1, MCSF
- Isotype
- Mouse IgG2b, κ
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Western blot analysis of 50 ng of recombinant human M-CSF (lane 1) and 50 ng of recombinant mouse M-CSF (lane 2) using anti-human M-CSF antibody (clone L1617F1).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 526002 | 500 µg | $347.00 | |||
M-CSF is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer. M-CSF is released by fibroblasts, breast cancer cell lines, alveolar macrophages, stromal bone marrow cells, endothelial cells, and mesenchymal cells. It binds CD14+ monocytes and promotes the survival/proliferation of human peripheral blood monocytes. In addition, M-CSF enhances inducible monocyte functions including phagocytic activity, microbial killing, cytotoxicity for tumor cells as well as synthesis of inflammatory cytokines such as IL-1, TNF-α, and IFN-γ in monocytes. M-CSF induces RANKL production in mature human osteoclasts and, consequently, this makes M-CSF a potent stimulator of mature osteoclast resorbing activity. Also, M-CSF induces VEGF production in human monocytes and human tumor cells. High levels of M-CSF, mononuclear phagocytes, and VEGF are associated with poor prognosis in patients with cancer.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human M-CSF
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2563444 (BioLegend Cat. No. 526002)
Antigen Details
- Structure
- Three isoforms with 256-554 amino acids, predicted molecular weight of approximately 30-70 kD.
- Distribution
-
Cell membrane; single-pass membrane protein. Processed macrophage colony-stimulating factor 1: secreted, extracellular space.
- Function
- Controls the production, differentiation, and function of macrophages.
- Interaction
- Homodimer or heterodimer, disulfide-linked. Interacts with CSF1R.
- Ligand/Receptor
- M-CSFR or CSF1R (CD115).
- Biology Area
- Cell Biology, Cell Proliferation and Viability, Immunology
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
1. Kawasaki ES, et al. 1985. Science 230:291.
2. Wei S, et al. 2010. J. Leuko. Biol. 88:495.
3. Hodge JM, et al. 2011. PloS One 6:e21462.
4. Morandi A, et al. 2011. PloS One 6:e27450.
5. Erblich B, et al. 2011. PloS One 6:e26317.
6. MacDonald KP, et al. 2010. Blood 116:3955. - Gene ID
- 1435 View all products for this Gene ID
- UniProt
- View information about M-CSF on UniProt.org
Other Formats
View All M-CSF Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human M-CSF | L1617F1 | WB |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-human M-CSF
Western blot analysis of 50 ng of recombinant human M-CSF (l...