- Clone
- QCRL-4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- ABC29, ABCC, GS-X, MRP, Multidrug resistance-associated protein 1, LTC4 transporter, leukotriene C(4) transporter
- Isotype
- Mouse IgG1, κ
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H69AR cells were treated with the True-Nuclear™ Transcription Factor Buffer Set and then intracellularly stained with purified MRP1 (clone QCRL-4) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 370402 | 100 µg | $141.00 | |||
MRP1 is a 190 kD integral membrane phosphoglycoprotein. It is a member of the human ATP-binding cassette transporter multidrug resistance protein1 (MRP1), encoded by ABCC1. MRP1 is expressed in most normal tissues, with higher levels in the testes, lung, skin, small intestine, colon, kidney, placenta, peripheral blood mononuclear cells, and skeletal muscle. ABCC1 encodes plasma membrane proteins that have important physiological transport functions and mediate a variety of xenobiotics against their concentration gradients. MRP1 plays an important role in the extrusion of drugs from the cell and their over expression can be a cause of failure of anticancer and antimicrobial chemotherapy.
Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- QCRL-4 was raised against membranes prepared from the human small cell lung cancer cell line, H69AR, which highly over expresses MRP1.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol.
- Application Notes
-
QCRL-4 detects a discontinuous conformation-dependent epitope wholly contained in NBD2 of MRP1. The epitope for QCRL-4 has been mapped to aa 1294-1531. QCRL-4 is reported to be an effective and specific inhibitor of MRP1 activity when used in transport assays with inside-out membrane vesicles. QCRL-1, QCRL-2, QCRL-3, and QCRL-4 each bind distinct non-overlapping epitopes.
NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended. - RRID
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AB_2566669 (BioLegend Cat. No. 370402)
Antigen Details
- Structure
- 190 kD integral membrane phosphoglycoprotein.
- Distribution
-
Testes, lung, skin, small intestine, colon, kidney, placenta, peripheral blood mononuclear cells, and skeletal muscle. Cytoplasm / plasma membrane.
- Function
- Extrusion of drugs from the cell.
- Biology Area
- Immunology
- Antigen References
-
1. Cole SP, et al. 2014. Annu. Rev. Pharmacol. Toxicol. 54:95.
2. Hipfner DR, et al. 1999. J. Biol. Chem. 274(22):15420.
3. Filipits M, et al. 1999. Anticancer Res. 6:5043.
4. Wright SR, et al. 1998. Clin. Cancer Res. 4:2279.
5. Hipfner DR, et al. 1998. Br. J. Cancer 78(9):1134.
6. Filipits M, et al. 1997. Leukemia 11(7):1073.
7. Loe DW, et al. 1997. Mol. Pharmacol. 6:1034-41.
8. Hipfner DR, et al. 1996. Cancer Res. 56:3307.
9. Filipits M, et al. 1996. Clin. Cancer Res. 2(7):1231.
10. Almquist KC, et al. 1995. Cancer Res. 55(1):102. - Gene ID
- 4363 View all products for this Gene ID
- UniProt
- View information about MRP1 on UniProt.org
Other Formats
View All MRP1 (ABCC1) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human/mouse MRP1 (ABCC1) | QCRL-4 | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-human/mouse MRP1 (ABCC1)
H69AR cells were treated with the True-Nuclear™ Transcriptio...