- Clone
- 1500102 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Insulin-Like Growth Factor 1, IGF-1, Mechano Growth Factor, Somatomedin-C, IGF-I, MGF, Insulin-Like Growth Factor IA, Insulin-Like Growth Factor IB, Nonsuppressible insulin-like activity I (NSILA-I)
- Isotype
- Mouse IgG1, κ
-
Recombinant human IGF-I (50ng) was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-IGF1 (clone 1500102) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 680402 | 100 µg | $276.00 | |||
IGF-I, also known as insulin-like growth factor 1), was initially described in 1957 as a hormone that is important for child development, and also has anabolic effects in adults. It is a member of the IGF family that is comprised of three members: IGF-I, IGF-II, and insulin. IGF-I and IGF-II share approximately 50% similarity with insulin at the amino acid level. Human IGF-I is synthesized as a 191 amino acid prepro-IGF-I; pro-IGF-I is processed to the mature, active protein, and this procedure requires glucose regulating protein 94 (GRP94) that possesses chaperone activity towards IGF-I. Deletion of muscle GRP94 impairs muscle and body growth by inhibiting local production of IGF proteins. Seven IGF binding proteins have been described (IGFBP-1 to -7). IGFBPs extend the half-lives and regulate the availability of IGF-I and IGF-II. It has been described that IGFBP3 is the main circulating binding protein for IGFs in serum. 75% of circulating IGF-I and IGF-II bind to IGFBP3 and the co-carrier acid labile subunit (ALS). IGF-I binds to three receptors, and the binding to IGFIR promotes cell proliferation, survival, differentiation, and migration. IGF-I deficiency is associated with growth hormone insensitivity syndrome (GHIS), which is also called Laron syndrome, liver cirrhosis, age-related cardiovascular, neurological diseases, and intrauterine growth restriction.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant Human IGF-I, amino acids (Gly49-Ala118) (Accession# PO05019), expressed in E. coli
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2566356 (BioLegend Cat. No. 680402)
Antigen Details
- Structure
- The 70 amino acid recombinant protein has a predicted molecular weight of approximately 7.6 kD. The predicted N terminal amino acid is Gly.
- Distribution
- Secreted protein.
- Function
- Similar to insulin in function and structure and is involved in mediating growth and development.
- Interaction
- IGF binding protein 1, IGF binding protein 2, IGF binding protein 3, IGF binding protein 4, IGF binding protein 5, IGF binding protein 6, and Fibronectin 1.
- Ligand/Receptor
- IGF1R.
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Signal Transduction
- Antigen References
-
1. Bell GI, et al. 1984. Nature 310:775.
2. Karey KP, Sirbasku DA. 1988. Cancer Res. 48:4083.
3. Mohan S, Baylink DJ. 2002. J. Endocrinol. 1:19.
4. Ostrovsky O, et al. 2010. Biochim. Biophys. Acta. 1803:333.
5. Barton ER, et al. 2012. FASEB. J. 26:3691.
6. Puche JE, Castilla-Cortazar I. 2012. J. Transl. Med. 10:224.
7. Ni F, et al. 2013. Nat. Commun. 4:1479. - Gene ID
- 3479 View all products for this Gene ID
- UniProt
- View information about IGF1 on UniProt.org
Other Formats
View All IGF1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-IGF1 | 1500102 | WB |
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Purified anti-IGF1
Recombinant human IGF-I (50ng) was resolved by electrophores...