- Clone
- LAT1111 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Linker for activation of T-cells, 36 kD phospho-tyrosine adaptor protein
- Isotype
- Mouse IgG1, κ
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Total lysates (15 µg protein) from HEL, U87-MG and Daudi (Low expressed cells) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/ml purified anti-LAT antibody, clone LAT1111. Proteins were visualized using chemiluminescence detection by incubation with and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 664804, 1:25,000 dilution). -
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| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 623901 | 25 µg | $101.00 | |||
| 623902 | 100 µg | $218.00 | |||
LAT (linker for activation of T-cells) is a 36 kD phospho-tyrosine adaptor protein containing SH2 domains and a CVRC motif. Two LAT isoforms have been reported with approximate molecular weights of 36 kD and 38 kD. LAT is localized to lipid rafts, and is also a potential type III transmembrane protein, thought to be expressed in the juxtanuclear intracellular compartment. LAT links TCR engagement to Ras-MAPK activation, Ca2+ flux, IL-2 expression and is required for T-cell development. LAT is phosphorylated when activated by TCR cross-linking, where it recruits signaling molecules. LAT activation can be prevented by polyunsaturated fatty acids. LAT is modified by ZAP-70 and Syk phosphorylation and can also be palmitoylated. LAT has been reported to be associated with Grb2, Gads, Grap, PLC-γ1, p85 subunit PI3K, ZAP-70, Syk, Itk, Vav, SLP-76, and cbl.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial recombinant protein (cytoplasmic domain of human LAT)
- Formulation
- This LAT antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store between 2°C and 8°C.
- Application
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WB - Quality tested
ICFC - Verified
IP - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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The LAT1111 monoclonal antibody recognizes the cytoplasmic domain of human LAT and has been shown to be useful for Western blotting and immunoprecipitation. The LAT1111 antibody does not cross-react with mouse LAT.
- Product Citations
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- RRID
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AB_2133118 (BioLegend Cat. No. 623901)
AB_2133118 (BioLegend Cat. No. 623902)
Antigen Details
- Structure
- SH2 domains, CVRC motif; two isoforms 36 kD, 38 kD
- Distribution
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Lipid rafts, potential type III transmembrane protein, juxtanuclear intracellular compartment
- Function
- Links TCR engagement to Ras-MAPK activation, Ca2+ flux, IL-2 expression; required for T-cell development
- Interaction
- Grb2, Gads, Grap, PLC-γ1, p85 subunit PI3K, ZAP-70, Syk, Itk, Vav, SLP-76, cbl
- Modification
- Phosphorylation by ZAP-70, Syk, Palmitoylated
- Cell Type
- Tregs
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Phospho-Proteins
- Antigen References
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1. Zhang W, et al. 1998. Cell. 92:83.
2. Zeyda M, et al. 2002. J. Biol. Chem. 277:28418.
3. Perez-Villar J, et al. 2002 Biochemistry. 41:10732.
4. Hartgroves L, et al. 2003 J. Biol. Chem. 278:20389. - Regulation
- Phosphorylated when activated by TCR cross-linking, recruits signaling molecules, activation prevented by polyunsaturated fatty acids
- Gene ID
- 27040 View all products for this Gene ID
- UniProt
- View information about LAT on UniProt.org
Other Formats
View All LAT Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-LAT | LAT1111 | WB,ICFC,IP |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-LAT
Total lysates (15 µg protein) from HEL, U87-MG and Daudi (Lo... 
