- Clone
- BL28598 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- LDHB
- Isotype
- Mouse IgG2b, κ
-
Western blot of purified anti-LDHB antibody (BL28598). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-LDHB antibody (clone BL28598) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm -
IHC staining of purified anti-LDHB antibody (clone BL28598) on formalin-fixed paraffin-embedded mouse kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm -
IHC staining of purified anti-LDHB antibody (clone BL28598) on formalin-fixed paraffin-embedded rat kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 859801 | 25 µg | $106.00 | |||
Lactate Dehydrogenase B (LDHB) is one of the subunits of the lactate dehydrogenase enzyme that catalyzes the synthesis of (S)-Lactate from pyruvate. LDHB was shown to be a downstream mediator of the mTOR signaling pathway that plays a critical role in hyperactive mTOR-mediated tumorigenesis, indicating it as potential therapeutic target for cancer and other diseases caused by aberrant mTOR signaling.
Product Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant Lactate Dehydrogenase B (LDHB) expressed in E.coli
- Formulation
- 0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application. For formalin-fixed paraffin-embedded immunohistochemical staining, a concentration range of 1 - 5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2810734 (BioLegend Cat. No. 859801)
Antigen Details
- Structure
- LDHB is a 334 amino acid protein with an apparent molecular mass of ~37 kD.
- Distribution
-
Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Cytosol - Function
- LDHB is part of the lactate synthesis pathway that synthesizes S-Lactate from pyruvate
- Interaction
- Forms Homotetramers
- Cell Type
- Mature Neurons
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers
- Molecular Family
- Enzymes and Regulators
- Antigen References
-
- Swiderek K and Paneth P. 2011. Arch. Biochem Biophys. 505(1):33
- Zha X, et al. 2011. Cancer Res. 71(1):13
- Gene ID
- 3945 View all products for this Gene ID 16832 View all products for this Gene ID 24534 View all products for this Gene ID
- UniProt
- View information about LDHB on UniProt.org
Other Formats
View All LDHB Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-LDHB | BL28598 | WB,IHC-P |
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