- Clone
- 14E6-4E6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Protein Lin-28 Homolog A,Lin-28A, CSDD1,ZCCHC1, Zinc Finger CCHC Domain-Containing Protein 1
- Isotype
- Mouse IgG2a, κ
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Total cell lysate from HeLa cells (lane 1, 15 µg), NTERA2 (lane 2, 15 µg) and NF1 (lane 3, 15 µg) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with purified anti-Lin28A antibody (clone 14E6-4E6). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control. -
Whole cell lysates (15 µg protein) from HeLa (negative control), HepG2 (negative control), NTERA-2, and HEL (negative control) and cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to a nitrocellulose membrane, and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-Lin28A antibody, clone 14E6-4E6. Proteins were visualized using chemiluminescence detection by incubating the membrane with a 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH antibody (1:2000 dilution, Cat. No. 649203) was used as a loading control (lower). Lane M: Molecular Weight Marker. -
NTERA‐2 cells were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X‐100 for 5 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 µg/mL Lin28A antibody (14E6‐4E6) and followed by DyLight™ 594 goat anti‐mouse IgG for one hour at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective. -
HeLa cells (negative target) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with 0.25 µg of purified anti-LIN28A antibody (clone 14E6-4E6) (filled histogram) or 0.25 µg of Purified Mouse IgG1, κ Isotype Ctrl Antibody (Cat. No. 400102) (open histogram) followed by PE goat anti-mouse IgG antibody (Cat. No. 405307). -
NTERA2 cells (positive target) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with 0.25 µg of purified anti-LIN28A antibody (clone 14E6-4E6) (filled histogram) or 0.25 µg of Purified Mouse IgG1, κ Isotype Ctrl Antibody (Cat. No. 400102) (open histogram) followed by PE goat anti-mouse IgG antibody (Cat. No. 405307)
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 657802 | 100 µg | $347.00 | |||
LIN28A is a highly conserved RNA-binding protein and reprogramming factor that plays important roles in development, self-renewal of stem cells, glucose metabolism and tumorigenesis. Lin28A modulates not only miRNAs, particularly the let-7 family miRNAs, but also the translation of mRNAs that are important for cell growth and metabolism. LIN28A/B has been observed to be overexpressed in a wide range of solid tumors and hematological malignancies, and has emerged as a new therapeutic target candidate for cancer.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human Lin28A from E. coli
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, ICFC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 1.0 µg per ml (1:500 - 1:20,000 dilution). For immunocytochemistry, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For flow cytometric staining, the suggested use of this reagent is 0.06 - 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
HepG2 and HEL cells were selected for specificity testing because they express LIN28B mRNA but lack detectable LIN28A expression (source: Human Protein Atlas).
This clone is specific for Lin28A does not recognize Lin28B. - RRID
-
AB_2562650 (BioLegend Cat. No. 657802)
Antigen Details
- Structure
- 209 amino acids, predicted molecular weight of 28 kD with the characteristic of a cold shock domain (CSD) and two tandem Cys-Cys-His-Cys (CCHC)-type zinc-binding motifs (CCHCx2)
- Distribution
-
Cytoplasm and nucleus; localizes to cytoplasmic processing bodies and stress granules
- Function
- Development, self-renewal of stem cells, glucose metabolism and tumorigenesis; regulates Let-7 family miRNA, translation of mRNA
- Interaction
- EIF3S2, NCL and ZCCHC11/TUT4
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, Immunology, Protein Synthesis, Signal Transduction, Stem Cells
- Antigen References
-
1. Shyh-Chang N, et al. 2013. Cell Stem Cell. 12:395.
2. Thornton JE, et al. 2012. Trends Cell Biol. 22:474.
3. Huang Y. 2012. Wiley Interdiscip. Rev. RNA. 3:483.
4. Cho J, et al. 2012. Cell. 151:765.
5. Li N, et al. 2012. J. Biol. Chem. 287:17386.
6. Zhu H, et al. 2011. Cell 147:81. - Gene ID
- 79727 View all products for this Gene ID
- UniProt
- View information about Lin28A on UniProt.org
Other Formats
View All Lin28A Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Lin28A | 14E6-4E6 | WB,ICC,ICFC |
| Alexa Fluor® 647 anti-Lin28A | 14E6-4E6 | ICFC |
| Alexa Fluor® 488 anti-Lin28A | 14E6-4E6 | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-Lin28A
Total cell lysate from HeLa cells (lane 1, 15 µg), NTERA2 (l... 
Whole cell lysates (15 µg protein) from HeLa (negative contr... 
NTERA‐2 cells were fixed with 4% paraformaldehyde (PFA) for ... 
HeLa cells (negative target) were fixed with Fixation Buffer... 
NTERA2 cells (positive target) were fixed with Fixation Buff... -
Alexa Fluor® 647 anti-Lin28A
HeLa cells (negative target) were fixed with Fixation Buffer... 
NTERA2 cells (positive target) were fixed with Fixation Buff... -
Alexa Fluor® 488 anti-Lin28A
HeLa cells (negative target) were fixed with Fixation Buffer... 
NTERA2 cells (positive target) were fixed with Fixation Buff...