Western blot of purified anti-Lysozyme antibody (Poly28600). Lane 1: Molecular weight marker; Lane 2: 20 µg of human spleen lysate. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
Western blot of purified anti-Lysozyme antibody (Poly28600). Lane 1: Molecular weight marker; Lane 2: 20 µg of human spleen lysate. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
Western blot of purified anti-Lysozyme antibody (Poly28600). Lane 1: Molecular weight marker; Lane 2: 10 µg of rat kidney lysate; Lane 3: 20 µg of mouse kidney lysate. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
IHC staining of purified anti-Lysozyme antibody (Poly28600) on formalin-fixed paraffin-embedded human spleen tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
IHC staining of purified anti-Lysozyme antibody (Poly28600) on formalin-fixed paraffin-embedded mouse kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
IHC staining of purified anti-Lysozyme antibody (Poly28600) on formalin-fixed paraffin-embedded rat kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Lysozyme is an antimicrobial enzyme that is abundantly present in secretions such as tears, saliva and mucus. Its hydrolase activity leads to the breakdown of the gram-positive bacterial cell wall and lysis of the bacteria.
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml for human, and 1.0 - 2.0 µg per ml for mouse and rat. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Lysozyme is expressed in many immune cells including monocytes and macrophages. It plays an important role in innate immunity by hydrolyzing peptidoglycans found in bacterial cell wall and thus destroying the bacteria.
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