Purified anti-MAP2 Antibody (Previously Covance catalog# SMI-52P)

Pricing & Availability
Clone
SMI 52 (See other available formats)
Regulatory Status
RUO
Other Names
Microtubule-associated protein 2, MAP-2
Previously
Covance Catalog# SMI-52P
Isotype
Mouse IgG1, κ
SMI-52_Pure_MAP2_Antibody_1_060118
IHC staining of purified anti-MAP2 antibody (clone SMI 52) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.16 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI-52_Pure_MAP2_Antibody_1_060118
    IHC staining of purified anti-MAP2 antibody (clone SMI 52) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.16 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI-52_Pure_MAP2_Antibody_2_060118
    IHC staining of purified anti-MAP2 antibody (clone SMI 52) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.16 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI-52_Pure_MAP2_Antibody_3_060118
    IHC staining of purified anti-MAP2 antibody (clone SMI 52) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.16 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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801810 25 µL $112.00
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801801 100 µL $275.00
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Description

Microtubules are 25nm diameter protein rods found in most kinds of eukaryote cells. They are polymerized from a dimeric subunit made of one α subunit and one β tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein τ (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5.

Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against Microtubule-Associated Protein 2 (MAP2).
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

IHC-P - Quality tested
ICC, ELISA, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.16 - 1.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for relevant formats) include: immunocytochemistry5,6, ELISA7, and WB1,5.

SMI 52 reacts with microtubule-associated protein 2 (MAP2). This antibody reacts with most mammalian species and with frog tissue. SMI 52 recognizes neuronal cell bodies and dendrites in tissue sections and cell cultures. In immunoblots of brain cytoskeletal preparations, SMI 52 reacts with MAP2a and b, and also with a doublet at 68 kD that may represent MAP2c.

Application References

(PubMed link indicates BioLegend citation)
  1. Shi Y, et al. 2017. Cell Death Differ. 24(1):167. (WB)
  2. Shelton MA, et al. 2015. Biol. Psychiatry. 78(6):374. (IHC) PubMed
  3. Wang X, et al. 2015. PLoS One. 10: 0145441. (IHC) PubMed
  4. Kazim SF, et al. 2014. Neuro Dis. 71:110. (IHC)
  5. Cardenas-Aguayo MC, et al. 2013. PLOS ONE. (1):e53596. (ICC, WB) PubMed
  6. Lo Furno D, et al. 2013. J. Cell. Physiol. 228:2109. (ICC)
  7. Gensel J, et al. 2009. J Neurosci. 29:3956-3968. (ELISA) PubMed
Product Citations
  1. Butler MLMD, et al. 2022. J Neuropathol Exp Neurol. 81:773. PubMed
  2. Ritzel RM, et al. 2023. Sci Adv. 9:eadd1101. PubMed
  3. Villar-Pazos S, et al. 2023. Nat Commun. 6547:14. PubMed
  4. Libanori A, et al. 2023. Adv Mater. 35:e2206933. PubMed
  5. McCann MM, et al. 2019. Journal of Comparative Neurology. 528(8):1293-1306. PubMed
  6. McKinney BC, et al. 2019. Neuropsychopharmacology. 44:1055. PubMed
  7. de Leeuw VC, et al. 2020. Cell Reprogram. 1.125. PubMed
  8. Stern AL et al. 2018. The Journal of Neuroscience. 38(18):4288-4300 . PubMed
  9. DeGiosio R, et al. 2019. NPJ Schizophr. 5:13. PubMed
  10. Rahman A et al. 2018. The Journal of comparative neurology. 527(4):797-817 . PubMed
  11. Gensel J, et al. 2009. J Neurosci. 29:3956-3968. PubMed
  12. Wang X, et al. 2015. PLoS One. 10: 0145441. PubMed
RRID
AB_2564643 (BioLegend Cat. No. 801810)
AB_2564643 (BioLegend Cat. No. 801801)

Antigen Details

Structure
MAP2 is made up of two bands of an apparent molecular weight of approximately 280 kD, referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kD on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternative transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein τ (tau).
Distribution

Cellular distribution: Plasma membrane, cytoskeleton, nucleus, and cytosol.
Tissue distribution: Predominantly in the nervous system

Function
MAP2 encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are involved in microtubule assembly. The products of these genes are neuron-specific cytoskeletal proteins that are enriched in dendrites, which implicate a role in determining and stabilizing dentritic shape during neuron development.
Interaction
MAP2 interacts with microtubules. It has also been reported to interact with KNDC1 via KIND2.
Ligand/Receptor
MAP2 isoforms are expressed in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites. In contrast τ (tau) is found predominantly in neuronal axons.
Cell Type
Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Microtubules
Antigen References
  1. Zhou Y, et al. 2015, Neuro Oncol. 17(12):1578.
  2. Loeser H, et al. 2015, Am J Nephrol. 41(3):191.
Gene ID
17756 View all products for this Gene ID 25595 View all products for this Gene ID
UniProt
View information about MAP2 on UniProt.org
Go To Top Version: 6    Revision Date: 07/09/2024

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