- Clone
- TUG8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Mouse Mast Cell Protease 8, Mcpt8
- Isotype
- Rat IgG2a, κ
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Cell extracts from untransfected NIH/3T3 cells (lane 1) or NIH/3T3 cells transfected with a plasmid encoding mMCP-8-Flag tagged protein (lane 2), using anti-mMCP-8, clone TUG8.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 647401 | 25 µg | $124.00 | |||
| 647402 | 100 µg | $293.00 | |||
mMCP-8 and its rat homologues, rMCP-8, -9, and -10, form a new group of mast cell/basophil proteases, which are more closely related to the T-cell granzymes and neutrophil cathepsin G than to the mast cell tryptases and chymases. mMCP-8 showed a high degree of homology with mouse granzyme B in the critical regions for determining substrate cleavage specificity. It preferentially cleaves after Asp residues. mMCP-8 mRNA is highly expressed in a mouse connective tissue MC-like tumor line. However, it could not be detected in mouse liver, intestine, lung or ears, indicating very low expression in normal tissues. A strong increase in mMCP-8 levels in the lungs can be detected in infected animals.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant GST-mouse mMCP-8 fusion protein expressed in E.coli
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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WB - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
The optimal concentration should be determined by titration for each individual assay of interest.
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Application References
(PubMed link indicates BioLegend citation) - Product Citations
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- RRID
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AB_2069309 (BioLegend Cat. No. 647401)
AB_2069309 (BioLegend Cat. No. 647402)
Antigen Details
- Structure
- A 247-aa polypeptide including a signal sequence of 18 aa followed by a 2-aa activation peptide (Gly-Glu) and a mature protease of 227 aa
- Distribution
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mMCP-8 was initially isolated as a cDNA from a mouse mast cell line, but has recently been found to be expressed primarily by mouse basophils.
- Cell Type
- Basophils, Mast cells
- Biology Area
- Cell Biology, Immunology
- Antigen References
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1. Lutzelschwab C, et al. 1998. Eur. J. Immunol. 28:1022.
2. Lunderius C, et al. 2001. Immunogenetics 53:225. - Gene ID
- 4179 View all products for this Gene ID
- UniProt
- View information about mMCP-8 on UniProt.org
Other Formats
View All mMCP-8 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mMCP-8 | TUG8 | WB |
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Purified anti-mMCP-8
Cell extracts from untransfected NIH/3T3 cells (lane 1) or N...