- Clone
- Poly28599 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MOG, Myelin Oligodendrocyte Glycoprotein
- Isotype
- Rabbit Polyclonal IgG
-
Western blot of purified anti-MOG antibody (Poly28599). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-MOG antibody (Poly28599) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 859901 | 25 µg | $125.00 | |||
Myelin Oligodendrocyte Glycoprotein (MOG) is a membrane protein expressed on the oligodendrocyte cell surface and the outermost surface of myelin sheaths. It is a primary target antigen involved in immune-mediated demyelination. This protein may be involved in completion and maintenance of the myelin sheath and in cell-cell communication, and mediate homophilic cell-cell adhesion. Diseases associated with MOG include Narcolepsy 7 and Rubella. Among its related pathways are Neural Stem Cell Differentiation Pathways and Lineage-specific Markers. MOG may also act as a receptor for rubella virus during microbial infection.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- Recombinant MOG expressed in E.coli.
- Formulation
- 0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2810735 (BioLegend Cat. No. 859901)
Antigen Details
- Structure
- MOG is a 247 amino acid protein with an apparent molecular mass of ~28 kD.
- Distribution
-
Tissue Distribution: Brain and spinal cord
Cellular Distribution: Plasma membrane - Function
- MOG is involved in completion and maintenance of the myelin sheath and cell-cell communication.
- Interaction
- Myelin, Rubella virus
- Cell Type
- Neurons, Oligodendrocytes
- Biology Area
- Cell Adhesion, Immunology, Neurodegeneration, Neuroscience, Stem Cells
- Molecular Family
- Adhesion Molecules, Innate Immune Signaling
- Antigen References
-
- Reindl M, et al. 2017. Curr Opin Neurol. 30(3):295.
- Hennes EM, et al. 2018. Neuropediatrics. 49(1):3.
- Gene ID
- 4340 View all products for this Gene ID 17441 View all products for this Gene ID 24558 View all products for this Gene ID
- UniProt
- View information about MOG on UniProt.org
Other Formats
View All MOG Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-MOG | Poly28599 | WB,IHC-P |
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