Purified anti-mouse CD19 Antibody

Pricing & Availability
Clone
1D3/CD19 (See other available formats)
Regulatory Status
RUO
Other Names
B4, CVID3
Isotype
Rat IgG2a, κ
1D3slashCD19_PURE_CD19_Antibody_080516
C57BL/6 mouse splenocytes were stained with purified CD19 (clone 1D3/CD19, filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG FITC.
  • 1D3slashCD19_PURE_CD19_Antibody_080516
    C57BL/6 mouse splenocytes were stained with purified CD19 (clone 1D3/CD19, filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG FITC.
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Cat # Size Price Quantity Check Availability
152402 500 µg $276.00
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Transfected k562 cells expressing the extracellular domain of murine CD19.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Block, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: blocking1,2 and immunoprecipitation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Krop I, et al. 1996. Eur. J. Immunol. 26:238. (FC, Blocking, IP)
  2. Abraham P, et al. 2014. Clin. Exp. Immunol. 175:181. (Blocking)
Product Citations
  1. Paterson MJ, et al. 2020. PLoS Pathog. 16:e1008733. PubMed
  2. Wolf Y, et al. 2017. J Exp Med. 214:905. PubMed
  3. Lei Y, et al. 2022. Comp Med. 72:93. PubMed
  4. Burrello C, et al. 2018. Front Med (Lausanne). 5:21. PubMed
  5. Dienz O, et al. 2020. J Immunol. 204:1521. PubMed
  6. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
  7. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  8. Dooley K, et al. 2021. Mol Ther. 29:1729. PubMed
  9. King IL, et al. 2017. Mucosal Immunol. 10:1160. PubMed
  10. Li J, et al. 2021. Front Immunol. 12:710406. PubMed
  11. Ye Y, et al. 2017. Sci Immunol. 2:17. PubMed
  12. Yoon BH, et al. 2018. Mol Cells. 41:953. PubMed
  13. Rive CM, et al. 2022. Mol Ther Methods Clin Dev. 26:4. PubMed
  14. Rosenbaum M, et al. 2019. Nat Commun. 2.05. PubMed
  15. Duncan CG, et al. 2018. G3 (Bethesda). 0.892361111. PubMed
  16. Liu J, et al. 2022. Stem Cell Res Ther. 13:247. PubMed
  17. Diao J, et al. 2018. J Immunol. 201:1306. PubMed
  18. Mincham KT, et al. 2021. Front Immunol. 11:601494. PubMed
RRID
AB_2629714 (BioLegend Cat. No. 152402)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD.
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells.

Function
Modulates B cell activation and differentiation.
Ligand/Receptor
CD21, CD81, Leu-13.
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org
Go To Top Version: 1    Revision Date: 08/09/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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