Purified anti-mouse CD226 (DNAM-1) Antibody

Pricing & Availability
Clone
10E5 (See other available formats)
Regulatory Status
RUO
Other Names
DNAM-1, PTA1 (platelet and T cell activation antigen 1), TLISA1, LFA-1 associated Molecule PTA-1
Isotype
Rat IgG2b, κ
10E5_PURE_CD226_Antibody_1_011718
C57BL/6 mouse splenocytes were stained with CD8a APC and purified CD226 (clone 10E5, left) or purified rat IgG2b, κ isotype control (right), followed by anti-rat IgG2b FITC.
  • 10E5_PURE_CD226_Antibody_1_011718
    C57BL/6 mouse splenocytes were stained with CD8a APC and purified CD226 (clone 10E5, left) or purified rat IgG2b, κ isotype control (right), followed by anti-rat IgG2b FITC.
  • 10E5_PURE_CD226_Antibody_2_011718
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml CD226 (clone 10E5) purified and CD3ε (clone 500A2) Alexa Fluor® 594 (green) overnight at 4°C, followed by 2.5 µg/ml of goat anti rat IgG (clone Poly4054) Alexa Fluor® 647 (red) for 2 hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
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128822 500 µg $370.00
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Description

CD226 (DNAM-1) is constitutively expressed on native CD8+ cells and on CD4+ T cells, macrophages and NK cells.This antibody (10E5) was reported to bind about 40% of inactivated CD4+ cells and binds only to differentiated Th1 cells, but not to Th2 or Th0 cells.It is also reported to suppress antigen-specific T cell expansion and EAE (experimental allergic encephalitis) mediated by Th1 cells.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Th1 polarized T cell clones
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Dardalhon V, et al. 2005. J. Immunol. 175:1558
  2. Huang Z, et al. 2011. J Immunol. 186:3421. PubMed
Product Citations
  1. Goh W, et al. 2020. Cell Rep. 33:108285. PubMed
  2. Wagner AK, et al. 2017. Nat Commun. 8:15627. PubMed
RRID
AB_2728146 (BioLegend Cat. No. 128822)

Antigen Details

Structure
65 kd glycoprotein of a member of Ig-superfamily containing 2 immunologlobulin-like domains.
Distribution

Expressed on majority of CD4+ and CD8+ T cells, NK cells, monocytes/macrophages and a subset of B cells and thymocytes. Reported to be specifically expressed on the surface of Th1 cells, but downregulated in Th2 cells.

Function
Mediates cellular adhesion, involved in LFA-1 costimulatory signal for T cell differentiation and proliferation
Ligand/Receptor
CD112 and CD155
Cell Type
B cells, Macrophages, Monocytes, NK cells, T cells, Th1
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1.Tahara Hanaoka S, et al. 2006. Blood 107:1491
2. Shibuya K, et al. 2003. J. Exp. Med. 198:1829

Gene ID
225825 View all products for this Gene ID
UniProt
View information about CD226 on UniProt.org
Go To Top Version: 0    Revision Date: 01/17/2018

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