- Clone
- S21003A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- GPA, CD235a, GYPA, Sialoglycoprotein alpha, MN sialoglycoprotein, PAS-2, Monoclonal antibody to mouse Glycophorin A (GYPA / CD235a)
- Isotype
- Rat IgG2b, κ
-
C57BL/6 mouse bone marrow cells were stained with anti-mouse CD45 FITC and purified anti-mouse CD235a (Glycophorin A) (clone S21003A) (left) or purified rat IgG2b, κ isotype control (right), followed by APC goat anti-rat IgG. Data shown was gated on RBCs and lymphoid together. -
C57BL/6 frozen mouse spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse CD235a (Glycophorin A) (clone S21003A) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 594 anti-rat IgG2b (red), 5 µg/mL of Alexa Fluor® 647 anti-mouse/human CD45R/B220 (blue), and Alexa Fluor® 488 anti-mouse CD3ε (green) (left) or Alexa Fluor® 488 anti-mouse F4/80 (green) (right) for two hours at room temperature. The images were captured by 10X objective. -
C57BL/6 frozen mouse submandibular gland section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse CD235a (Glycophorin A) (clone S21003A) (left) or 5% FBS only (right) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 594 anti-rat IgG2b (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The images were captured by 10X objective.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 116702 | 100 µg | $317.00 | |||
CD235a, also known as Glycophorin A (Gypa) is a type I transmembrane sialoglycoprotein that is expressed on mouse erythrocytes, erythroid precursor cells and certain leukemic cells. Glycophorin A has been proposed to be involved in translocation of SLC4A1 to the plasma membrane. Glycophorin A may also be involved in regulation of response to osmotic stress.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse Glycophorin A-transfectant cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone S21003A does not block the binging of clone Ter-119 to target cells.
Clone S21003A could induce RBCs aggregation at high concentration.
Clone S21003A reacts with mouse CD235a (Glycophorin A). - Product Citations
-
- RRID
-
AB_2924454 (BioLegend Cat. No. 116702)
Antigen Details
- Structure
- CD235a is type I transmembrane sialoglycoprotein
- Distribution
-
Expressed on mouse erythrocytes, erythroid precursor cells and certain leukemic cells
- Function
- Regulation of response to osmotic stress. May be involved in translocation of SLC4A1 to the plasma membrane
- Cell Type
- Erythrocytes
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
- Arimitsu N, et al. 2003. Genes Cells. 8:769-777-77.
- Devin RB, et al. 2016. Proc Natl Acad Sci USA. 113:5245-50.
- Gene ID
- 14934 View all products for this Gene ID
- UniProt
- View information about CD235a on UniProt.org
Other Formats
View All CD235a Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse CD235a (Glycophorin A) | S21003A | FC,IHC-F |
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