- Clone
- W20119E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- EGP40, MIC18, TROP1, KSA
- Isotype
- Rat IgG2b, κ
-
TE-71 cells were stained with purified anti-mouse CD326 (Ep-CAM) (clone W20119E) (filled histogram) or purified rat IgG2b, κ isotype control (open histogram) followed by PE anti-rat IgG. -
C57 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse CD326 (Ep-CAM) (clone W20119E) and Alexa Fluor® 647 anti-mouse Podoplanin (clone PMab-1) (green) at 4°C overnight. On the next day, tissue was incubated with Alexa Fluor® 594 goat anti-rat IgG2b (clone MRG2b-85) (red) for an hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X object.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 112202 | 100 µg | $105.00 | |||
Ep-CAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of Ep-CAM seems to correlate inversely with the level of E-cadherin (CD324). Ep-CAM is considered important in tumor biology.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Verified
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2924452 (BioLegend Cat. No. 112202)
Antigen Details
- Structure
- 40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
- Distribution
-
Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types
- Function
- Mediates calcium-independent homophilic cell-cell adhesion
- Interaction
- CD326 displays hemophilic binding.
- Ligand/Receptor
- CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM
- Antigen References
-
- Borkowski TA, et al. 1996. Eur J Immunol. 26:110-4.
- Bergsagel PL, et al. 1992. J Immunol. 148:590-6.
- Gene ID
- 17075 View all products for this Gene ID
- UniProt
- View information about CD326 on UniProt.org
Other Formats
View All CD326 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse CD326 (Ep-CAM) | W20119E | FC,IHC-F |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-mouse CD326 (Ep-CAM)
TE-71 cells were stained with purified anti-mouse CD326 (Ep-... 
C57 mouse frozen kidney section was fixed with 4% paraformal...