Purified anti-mouse CD326 (Ep-CAM) Antibody

Pricing & Availability
Clone
W21115A (See other available formats)
Regulatory Status
RUO
Other Names
CD326, EGP40, MIC18, TROP1, KSA
Isotype
Rat IgG2a, κ
a. W21115A_PURE_CD326_IHC-F_092423
IHC staining of purified anti-mouse CD326 (Ep-CAM) (clone W21115A) on frozen mouse uterus section. C57BL/6 mouse frozen uterus section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the tissue was incubated without (panel A) or with (panel B) 5 µg/mL of purified anti-mouse CD326 (Ep-CAM) at 4°C overnight, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. The nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective. Scale bar: 50 µm
  • a. W21115A_PURE_CD326_IHC-F_092423
    IHC staining of purified anti-mouse CD326 (Ep-CAM) (clone W21115A) on frozen mouse uterus section. C57BL/6 mouse frozen uterus section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the tissue was incubated without (panel A) or with (panel B) 5 µg/mL of purified anti-mouse CD326 (Ep-CAM) at 4°C overnight, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. The nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective. Scale bar: 50 µm
  • b. W21115A_PURE_CD326_IHCP_092523
    IHC staining of purified anti-mouse CD326 (Ep-CAM) (clone W21115A) on formalin-fixed paraffin-embedded C57BL/6 mouse intestine tissue. Following antigen retrieval using 1X citrate buffer 1X (10 mM, pH 6.0) (Cat. No. 420901), the tissue was incubated without (panel A) or with (panel B) 1 µg/mL of purified anti-mouse CD326 (Ep-CAM) at 4°C overnight, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. The nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective. Scale bar: 50 µm
Cat # Size Price Quantity Check Availability
114202 100 µg $375.00
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Description

EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein aa24-266
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-F - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemical staining on frozen tissue sections, a concentration range of 2.5 - 10.0 µg/mL is suggested. For formalin-fixed paraffin-embedded immunohistochemical staining, a concentration range of 1.0 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3097223 (BioLegend Cat. No. 114202)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types

Function
Mediates calcium-independent homophilic cell-cell adhesion
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Borkowski TA, et al. 1996. Eur J Immunol. 26:110-4.
  2. Bergsagel PL, et al. 1992. J Immunol. 148:590-6.
Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org
Go To Top Version: 1    Revision Date: 08/18/2023

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