Purified anti-mouse CD34 Antibody

Pricing & Availability
Clone
MEC14.7 (See other available formats)
Regulatory Status
RUO
Other Names
Mucosialin
Isotype
Rat IgG2a, κ
MEC14-7_Pure_122607
Mouse NIH/3T3 cell line stained with purified MEC14.7, followed with biotinylated anti-rat IgG and Sav-PE
  • MEC14-7_Pure_122607
    Mouse NIH/3T3 cell line stained with purified MEC14.7, followed with biotinylated anti-rat IgG and Sav-PE
  • MEC14-7_Purified_CD34_Antibody_IF_012914
    NIH3T3 cells were fixed with 1% PFA and then stained with 10 µg/1ml purified CD34 (clone MEC14.7) for 3 hours at room temperature. The cells were then stained with 2.5 mg/ml anti-rat IgG Dylight™ 594 secondary antibody (red) for 1 hours at room temperature. The nuclei were counterstained with DAPI and are shown in blue. The image was captured by 40X objective.
  • MEC14dot7_PURE_CD34_Antibody_IHC-F_062118
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of purified anti-mouse CD34 (clone MEC14.7) and anti-mouse CD3ε (clone 500A2) Alexa Fluor® 594 (green) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 647 goat anti-rat IgG (clone Poly4054, red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
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119301 50 µg $71.00
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119302 500 µg $293.00
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Description

CD34 is a highly glycosylated hematopoietic progenitor antigen. Two isoforms of CD34 have been reported to be generated by alternative splicing. This antigen is expressed on hematopoietic progenitors as well as on endothelial cells, brain, and testis. CD34 is thought to function as an adhesion molecule for early hematopoietic progenitors mediating the attachment of stem cells to extracellular matrix or stromal cells. CD34 is phosphorylated on serine residues by PKC.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Cells transfected with mouse CD34
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, IHC-F - Verified
WB, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The MEC14.7 antibody does not stain bone marrow cells like some other mouse CD34 antibodies, probably because the antibody recognizes a different epitope from other mAbs. Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting6, and immunohistochemistry of acetone-fixed frozen sections and paraffin-embedded sections2,4,5,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Garlanda C, et al. 1997. Eur. J. Cell Biol. 73:368. (FC)
  2. Knowles HJ, et al. 2004. Circ. Res. 95:162. (IHC)
  3. Trempus CS, et al. 2003. J. Invest. Dermatol. 120:501.
  4. Winding B, et al. 2002. Clin. Cancer Res. 8:1932. (IHC)
  5. Voswinckel R, et al. 2003. Circ. Res. 93:372. (IHC)
  6. Kairaitis LK, et al. 2005. Am. J. Physiol. Renal. Physiol. 288:F198. (IHC, WB)
  7. Ao A, et al. 2008. P. Natl. Acad. Sci. USA 105:7821. PubMed
  8. Zaynagetdinov R., et al. 2011. J Immunol. 187:5703. PubMed.
Product Citations
  1. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  2. Zhang J, et al. 2018. Am J Physiol Renal Physiol. 315:F852. PubMed
  3. Blackshear CP, et al. 2018. Tissue Eng Part C Methods. 24:566. PubMed
  4. Uhlik M, et al. 2016. Cancer Res . 76: 2573 - 2586. PubMed
  5. Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed
  6. Abe SI, et al. 2017. PLoS One. 12:e0188705. PubMed
  7. Zhao J, et al. 2015. PLoS One. 10: 0131674. PubMed
  8. Lin XJ, et al. 2018. Mol Ther Nucleic Acids. 9:1127. PubMed
  9. Zaynagetdinov R, et al. 2011. J Immunol. 187:5703. PubMed
  10. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  11. Tran NL, et al. 2022. Elife. 11:. PubMed
RRID
AB_345279 (BioLegend Cat. No. 119301)
AB_345279 (BioLegend Cat. No. 119302)

Antigen Details

Structure
Type I membrane protein, 75-120 kD, highly glycosylated; two isoforms reported
Distribution

Hematopoietic progenitors, brain, testis, endothelial cells; low expression in thymus, spleen, and bone marrow

Function
Possible adhesion molecule thought to function in early hematopoiesis by mediating attachment of stem cells to bone marrow extracellular matrix or stromal cells. Presents carbohydrate ligands to selectins.
Ligand/Receptor
L-selectin, other selectins
Cell Type
Endothelial cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Garlanda C, et al. 1997. Eur. J. Cell Biol. 73:368.
2. Brown J, et al. 1991. Int. Immunol. 3:175.
3. Suda J, et al. 1992. Blood 79:2288.
4. Baumhueter S, et al. 1994. Blood 84:2554.

Gene ID
12490 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org
Go To Top Version: 3    Revision Date: 02/18/2014

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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