Purified anti-mouse CD355 (CRTAM) Antibody

Pricing & Availability
Clone
11-5/CRTAM (See other available formats)
Regulatory Status
RUO
Other Names
Class I-restricted T cell-associated molecule
Isotype
Rat IgG2a, κ
11-5CRTAM_Pure_1_080911
C57BL/6 mouse splenocytes were stimulated with immobilized TCR-β (clone H57-597), then stained with CD8 Alexa Fluor® 647 and purified CD355 (clone 11-5/CRTAM) (top) or rat IgG2a, κ isotype control (bottom), followed by anti-rat IgG FITC.
  • 11-5CRTAM_Pure_1_080911
    C57BL/6 mouse splenocytes were stimulated with immobilized TCR-β (clone H57-597), then stained with CD8 Alexa Fluor® 647 and purified CD355 (clone 11-5/CRTAM) (top) or rat IgG2a, κ isotype control (bottom), followed by anti-rat IgG FITC.
  • 11-5CRTAM_Pure_2_080911
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Cat # Size Price Quantity Check Availability
142002 100 µg $282.00
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Description

Class-I MHC-restricted T-cell associated molecule (CRTAM), designated as CD355, is an Ig domain like type I transmembrane receptor, which belongs to the nectin family. In the immune system, CRTAM is strictly expressed on activated NK cells and activated CD8-positive T cells. By binding its ligand Nectin-like molecule 2 (Necl2), CRTAM plays an important role in mediating cell adhesion and migration.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CRTAM extracellular region-human IgG Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for relevant formats) include:  Western blotting1.

Application References

(PubMed link indicates BioLegend citation)
  1. Arase N, et al. 2005. Int. Immunol. 17:1227. (WB)
RRID
AB_10899412 (BioLegend Cat. No. 142002)

Antigen Details

Structure
75 kD homodimer, nectin family, Ig superfamily
Distribution

Activated NK cells and activated CD8-positive T cells

Function
Cell adhesion, migration
Ligand/Receptor
Nectin-like molecule 2
Cell Type
NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Takeuchi A, et al. 2009. J. Immunol. 183:4220.
2. Yeh JH, et al. 2008. Cell 132:846.
3. Arase N. 2005. Int. Immunol. 17:1227.
4. Boles KS, et al. 2005. Blood 106:779.
5. Galibert L, et al. 2005 J. Biol. Chem. 280:21955.
6. Kennedy J, et al. 2000 J. Leukoc. Biol. 67:725.

Gene ID
54698 View all products for this Gene ID
UniProt
View information about CD355 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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