- Clone
- 3B3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T cell immunoglobulin and mucin domain containing protein-1, T cell and airway phenotype regulator (Tapr), hepatitic virus cellular receptor 1, CD365
- Isotype
- Rat IgG2a, κ
-
Mouse Tim-1 transfected cells were stained with purified anti-mouse CD365 (clone 3B3, filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 151702 | 100 µg | $211.00 | |||
CD365 (Tim-1) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-1 and hepatitis virus cellular receptor 1. It is developmentally expressed at high levels in the blastocyst. Tim-1 is expressed on activated CD4+ lymphocytes especially on Th2 cells and has been implicated to play a critical role in the development of atopic disease and other Th2-biased immune responses. Tim-1 is hepatitis A virus receptor in humans. Tim-4 is the endogenous ligand of Tim-1. The interaction of Tim-1 and Tim-4 is involved in costimulation of T cell proliferation. Tim-1 is an endogenous ligand for LMIR5/CD300b.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse TIM-1 (igV domain) FC chimera IFA
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
Direct ELISA - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.75 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- RRID
-
AB_2616785 (BioLegend Cat. No. 151702)
Antigen Details
- Structure
- Transmembrane protein containing immunoglobulin domain and mucin-like domain with the predicted molecular weight of 33 kD.
- Distribution
-
Developmentally regulated; highly expressed in embryonic development in blastocysts. Expressed on activated CD4+ lymphocytes, especially Th2 cell lines.
- Function
- Binds hepatitis virus, may play a critical role in immune responses and the development of atopic diseases (in particular airway hyperreactivity).
- Ligand/Receptor
- Binds hepatitis A virus in humans, Tim-4, and LMIR5/CD300b.
- Biology Area
- Cell Biology, Cell Proliferation and Viability, Immunology
- Molecular Family
- CD Molecules, TCRs
- Antigen References
-
1. McIntire JJ, et al. 2001. Nat. Immunol. 2:1109.
2. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
3. Wills-Karp M, et al. 2001. Nat. Rev. Immunol. 1:69.
4. Meyers JH, et al. 2005. Nat. Immunol. 6(5):455.
5. Umetsu S, et al. 2005. Nat. Immunol. 6:447.
6. Lee HH, et al. 2010. J. Immunol. 185:5225. - Gene ID
- 171283 View all products for this Gene ID
- UniProt
- View information about CD365 on UniProt.org
Other Formats
View All CD365 (Tim-1) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse CD365 (Tim-1) | 3B3 | FC,Direct ELISA |
| TotalSeq™-A1013 anti-mouse CD365 (Tim-1) | 3B3 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-mouse CD365 (Tim-1)
Mouse Tim-1 transfected cells were stained with purified ant... -
TotalSeq™-A1013 anti-mouse CD365 (Tim-1)