Purified anti-mouse CD40 Antibody

Pricing & Availability
Clone
3/23 (See other available formats)
Regulatory Status
RUO
Other Names
Bp50, TNFRSF5
Isotype
Rat IgG2a, κ
1_3slash23_Pure_021009.jpg
BALB/c mouse splenocytes stained with purified 3/23, followed by anti-rat IgG PE
  • 1_3slash23_Pure_021009.jpg
    BALB/c mouse splenocytes stained with purified 3/23, followed by anti-rat IgG PE
  • 2_3-23_PURE_CD40_Antibody_2_1012118
    Balb/C mouse frozen spleen tissue slices were fixed with 4% PFA for ten minutes and blocked with 5% FBS for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-mouse CD40 antibody (clone 3/23) overnight at 4°C. On the next day, tissue was incubated with Alexa fluor® 647 Goat anti-rat IgG (clone Poly4054, red) and Alexa Fluor® 594 anti-mouse CD3ε Antibody clone 500A2, green) for an hour at room temperature. Nuclei were counter-stained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
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124601 50 µg $71.00
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124602 500 µg $273.00
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Description

CD40 is a 48 kD type I transmembrane glycoprotein also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD40 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 124627 &124628) with a low endotoxin limit (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Hasbold J, et al. 1994. Eur. J. Immunol. 24:1835.
  2. Bourgeois C, et al. 2002. Science 297:2060.
Product Citations
  1. Cai S, et al. 2020. Sci Rep. 10:14249. PubMed
  2. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  3. Yamane M, et al. 2020. FASEB J. 34:10778. PubMed
  4. Song K, et al. 2017. Oncotarget. 8:38554. PubMed
  5. Diao J, et al. 2018. J Immunol. 201:1306. PubMed
  6. Bessell CA, et al. 2020. JCI Insight. 5:. PubMed
RRID
AB_1134239 (BioLegend Cat. No. 124601)
AB_1134239 (BioLegend Cat. No. 124602)

Antigen Details

Structure
48 kD type I transmembrane glycoprotein, a member of the tumor necrosis factor receptor (TNFR) superfamily.
Distribution

Expressed on B lymphocytes and other antigen-presenting cells, such as macrophages, follicular dendritic cells, etc.

Function
Regulates B cell growth and differentiation and Ig isotype switching. Interaction of CD40 with its ligand, CD154, on activated T cells is important for immune response.
Ligand/Receptor
CD154 on T cells
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, Macrophages
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Grewal IS, et al. 1998. Annu Rev Immunol 16:111.

Gene ID
21939 View all products for this Gene ID
UniProt
View information about CD40 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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