Purified anti-mouse CD40 Antibody

Pricing & Availability
Clone
1C10 (See other available formats)
Regulatory Status
RUO
Other Names
Bp50, TNFRSF5
Isotype
Rat IgG2a, κ
1C10_Purified_080607
C57BL/6 mouse splenocytes stained with purified 1C10, followed by anti-rat IgG FITC
  • 1C10_Purified_080607
    C57BL/6 mouse splenocytes stained with purified 1C10, followed by anti-rat IgG FITC
Cat # Size Price Quantity Check Availability
102802 500 µg $235.00
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Description

CD40 is a 48 kD type I transmembrane glycoprotein, also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Costim, Block, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 1C10 antibody has been reported to stimulate B cell proliferation and block CD40-CD40L interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation, blocking5,6 of CD40 ligand binding, and in vitro stimulation of B cell proliferation1-3. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102810). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102812) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Ferlin WG, et al. 1998. Eur. J. Immunol. 28:525. (FC, Costim)
  2. Ferlin WG, et al. 1996. Eur. J. Immunol. 26:2911. (Costim)
  3. Heath A, et al. 1994. Eur. J. Immunol. 24:1828. (Costim)
  4. Ashman RF, et al. 2005. Int. Immunol. 17:411.
  5. Pelßez B, et al. 2001. J. Immunol. 166:6608. (Block)
  6. Zhang P, et al. 2007. J. Immunol. 178:474. (Block)
  7. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  8. Ponomarev ED, et al. 2007. J.Immunol.178:39. PubMed
Product Citations
  1. Chi H, et al. 2022. Cells. 11: . PubMed
  2. Obieglo K, et al. 2019. Eur J Immunol. 49:1226. PubMed
  3. Zhang P, et al. 2007. J Immunol. 178:474. PubMed
  4. Prado C, et al. 2021. J Neuroinflammation. 18:292. PubMed
  5. Zhang Y, et al. 2020. Front Immunol. 11:1012. PubMed
  6. Ashman R, et al. 2005. Int Immunol. 0.99375. PubMed
  7. Hoves S, et al. 2018. J Exp Med. 215:859. PubMed
  8. Le Q, Maizels N 2015. PLoS One. 11: 1005411. PubMed
  9. Patel J, et al. 2022. Int J Biol Sci. 18:2670. PubMed
  10. Sakaguchi T, et al. 2020. Cell Reports. 31(10):107755. PubMed
  11. Graf R et al. 2019. Cell reports. 26(5):1098-1103 . PubMed
  12. Lu X, et al. 2019. Cell Rep. 28:472. PubMed
  13. Rastelli J, et al. 2008. Blood. 111:1448. PubMed
  14. Khameneh HJ, et al. 2017. J Immunol. 198:196. PubMed
RRID
AB_312935 (BioLegend Cat. No. 102802)

Antigen Details

Structure
TNFR superfamily, 48 kD
Distribution

B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, CD34+ hematopoietic progenitors

Function
Stimulates B cell growth, differentiation and isotype switching, stimulates macrophage and dendritic cell cytokine production
Ligand/Receptor
CD40L (CD154)
Cell Type
B cells, Dendritic cells, Endothelial cells, Epithelial cells, Hematopoietic stem and progenitors, Macrophages
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881.
3. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494.

Gene ID
21939 View all products for this Gene ID
UniProt
View information about CD40 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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